Dietary supplementation of glutamine on growth performance, intestinal morphology, hematic, enzymatic and immunological responses of the Nile Tilapia subjected to bacterial challenge

• Grant number: 14/01973-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: July 01, 2014
• End date: February 02, 2017
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Margarida Maria Barros
• Grantee: Pedro Luiz Pucci Figueiredo de Carvalho
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 15/23777-6 - Dietary supplementation of glutamine on growth performance, intestinal morphology, hematic, enzymatic and immunological responses of the Nile tilapia subjected to bacterial challenge, BE.EP.DR

Abstract

Two experiments will be conducted to evaluate the effect of glutamine supplementation in Nile tilapia diets. Study I: aims to determine the optimal level of glutamine supplementation through productive performance responses, body composition, haematological, immunological, biochemical responses and intestinal morphology. Four hundred masculinized Nile tilapia juveniles (5g) will be distributed in 40 tanks (250 L each), in a recirculating water system. The experimental design will be completely randomized with five treatments (0, 0.5, 1.0, 1.5 and 2.0 % glutamine ) and eight replicates. The fish will be hand fed four times a day for 90 days. Study II: the contribution of glutamine supplementation in haematological, immunological and biochemical responses will be assessed after bacterial challenge by Aeromonas hydrophila. After the productive performance test, 60 fish (150 g) will be inoculated intraperitoneally with pathogen and transferred to experimental challenge room containing 30 tanks of 40 liters each, making six replications per treatment in a completely randomized design. At the end of 15 days, haematological, immunological and biochemical parameters of these animals will be analyzed. The data will be submitted to analysis of variance. In case of differences (P < 0.05), will be subjected to regression analysis and compared using the Tukey test. Variables that did not follow normal distribution will be submitted to the Kruskal - Wallis test, complemented by the Mann-Whitney test. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARVALHO, PEDRO L. P. F.; YAMAMOTO, FERNANDO Y.; BARROS, MARGARIDA M.; GATLIN, III, DELBERT M.. L-glutamine in vitro supplementation enhances Nile tilapia Oreochromis niloticus (Linnaeus, 1758) leukocyte function. FISH & SHELLFISH IMMUNOLOGY, v. 80, p. 592-599, SEP 2018. (15/23777-6, 14/01973-5)

CARVALHO, PEDRO L. P. F.; XAVIER, WILLIAM DOS S.; GUIMARAES, MATHEUS G.; RODRIGUES, EDGAR J. D.; FURUYA, WILSON M.; YAMAMOTO, FERNANDO Y.; PEZZATO, LUIZ E.; GATLIN III, DELBERT M.; BARROS, MARGARIDA M.. Dietary glutamine improves growth and intestinal morphology of juvenile GIFT tilapia (Oreochromis niloticus) but has limited effects on innate immunity and antioxidant capacity. Aquaculture, v. 563, p. 9-pg., 2023-01-30. (14/01973-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARVALHO, Pedro Luiz Pucci Figueiredo de. Dietary glutamine supplementation on growth performance, intestinal morphology, hematic, enzymatic and immunological responses of Nile tilapia juveniles subjected to bacterial challenge. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.