Use of genotyping-by-sequencing (GBS) for the identification of molecular markers SNP (single nucleotide polymorphism) and construction of the linkage map in Hevea brasiliensis

• Grant number: 14/11807-5
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): August 01, 2014
• Effective date (End): February 29, 2016
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Anete Pereira de Souza
• Grantee: Luciano Henrique Braz dos Santos
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The rubber tree, species originating from the Amazon has a great economic importance, being a major source of natural rubber in the world, which serves as raw material for the most diverse products, among which stand out products used in hospitals and tires. Having large planted area throughout the world, about 95% of these areas use clones originating of 5 to 10 primary trees, thus depending on a close genetic basis. Having a long cycle improvement of about 30 years the use of new techniques can represent a decrease this time. In this context, emerge the molecular markers SNPs (single nucleotide polymorphism), which can assist breeding programs of rubber. In this context the use of a recently described technique, known as genotyping-by-sequencing (GBS), allows for the discovery of SNPs in large quantities, and using these data, it is possible to obtain high resolution genetic maps which can help the discovery of chromosomal regions that control characters with continuous pattern of phenotypic variation, known as QTL (Quantitative Trait Loci). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CONSON, ANDRE R. O.; TANIGUTI, CRISTIANE H.; AMADEU, RODRIGO R.; ANDREOTTI, ISABELA A. A.; DE SOUZA, LIVIA M.; DOS SANTOS, LUCIANO H. B.; ROSA, JOAO R. B. F.; MANTELLO, CAMILA C.; DA SILVA, CARLA C.; SCALOPPI JUNIOR, ERIVALDO JOSE; et al. High-Resolution Genetic Map and QTL Analysis of Growth-Related Traits of Hevea brasiliensis Cultivated Under Suboptimal Temperature and Humidity Conditions. FRONTIERS IN PLANT SCIENCE, v. 9, AUG 24 2018. (15/24346-9, 12/50491-8, 14/11807-5, 12/05473-1, 17/07908-9, 14/18755-0)

DE SOUZA, LIVIA M.; DOS SANTOS, LUCIANO H. B.; ROSA, JOAO R. B. F.; DA SILVA, CARLA C.; MANTELLO, CAMILA C.; CONSON, ANDRE R. O.; SCALOPPI, JR., ERIVALDO J.; FIALHO, JOSEFINO DE F.; DE MORAES, MARIO LUIZ T.; GONCALVES, PAULO DE S.; et al. Linkage Disequilibrium and Population Structure in Wild and Cultivated Populations of Rubber Tree (Hevea brasiliensis). FRONTIERS IN PLANT SCIENCE, v. 9, JUL 3 2018. (12/50491-8, 14/11807-5, 17/07908-9, 14/18755-0, 12/05473-1)