Inflammatory activation induced by sleep deprivation: possible link between sleep and impairment of lipid metabolism

• Grant number: 14/10255-9
• Support type: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): September 01, 2014
• Effective date (End): August 31, 2017
• Field of knowledge: Biological Sciences - Physiology - General Physiology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal researcher: Monica Levy Andersen
• Grantee: Paula Cristina Alves Araújo
• Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated scholarship(s): 15/07354-8 - Role of NLRP3 in immune activation induced by sleep deprivation, BE.EP.PD

Abstract

Growing evidence suggest a link between short sleep duration and cardiometabolic diseases. One possible mechanism is the proinflammatory process induced by sleep loss. We will explore the hypothesis that sleep deprivation induces cellular stress, which triggers activation of inflammatory responses, specifically the NLRP3 signaling pathways, leading to up-regulation of inflammatory markers and down-regulation of lipid transport pathways. Adult male mice (wild type, Nlrp3-/-) will be sleep deprived for 3 or 6 hours. After each total sleep deprivation period or undisturbed sleep (home-cage control), animals will be euthanized and brain, blood and liver tissues will be collected. The tissues will be analyzed for gene expression of lipid metabolism and transport pathways (Abca1, Abcg1, Srebf1, Srebf2 genes) as well as inflammation-related genes (Tlr4, Nfkb, Tnf-±, Il-1b, Il-18, Il-12, Il-6, iNOS, Ccl2, COX2, Stat-3, Stat-4). During the experiment, the spontaneous sleep-wake cycle as well as sleep deprivation and sleep opportunity after the deprivation will be monitored using EEG/EMG recording. Understanding the mechanisms by which sleep deprivation induces activation of the immune responses and metabolic changes could help us to understand the relation between sleep loss and adverse health effects. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GALANJAUSKAS, HELOISA; ARAUJO, PAULA; TUFIK, SERGIO; ANDERSEN, MONICA L.. Sleep disturbance as a stressor event in elderly's life. NEUROBIOLOGY OF AGING, v. 60, p. 203-204, DEC 2017. (14/10255-9)

FERNANDES, GUILHERME L.; ARAUJO, PAULA; TUFIK, SERGIO; ANDERSEN, MONICA L.. The role of IL-6 and STAT in sleep and neuroinflammation. Clinical Immunology, v. 180, p. 58-59, JUL 2017. (14/10255-9)

BERTI, P. H.; ARAUJO, P.; TUFIK, S.; ANDERSEN, M. L.. Impact of sleep disorders in cognitive function of thyroid cancer survivors. SUPPORTIVE CARE IN CANCER, v. 25, n. 9, p. 2675-2676, SEP 2017. (14/10255-9)

KIM, LENISE JIHE; COELHO, FERNANDO MORGADINHO; HIROTSU, CAMILA; ARAUJO, PAULA; BITTENCOURT, LIA; TUFIK, SERGIO; ANDERSEN, MONICA LEVY. Frequencies and Associations of Narcolepsy-Related Symptoms: A Cross-Sectional Study. JOURNAL OF CLINICAL SLEEP MEDICINE, v. 11, n. 12, p. 1377-1384, 2015. (14/10255-9, 13/14420-1, 14/15259-2)

KIM, LENISE JIHE; COELHO, FERNANDO MORGADINHO; ARAUJO, PAULA; TEDESCO, ROBERTO CARLOS; SOUZA, RODRIGO BARBOSA; TUFIK, SERGIO; ANDERSEN, MONICA LEVY. Sleep restriction reduces the survival time and aggravates the neurological dysfunction and memory impairments in an animal model of cerebral hypoperfusion. Brain Research, v. 1644, p. 213-221, AUG 1 2016. (13/14420-1, 14/10255-9)