Antibacterial agents are crucial drugs for life expectancy. Several reasons justify the need by new antibacterials, including high bacterial resistance and mortality caused by infectious diseases. Thus, efforts are urgent to develop innovative antibacterial drugs. Recently, curcumin and cinnmaldehyde exhibited potent antibacterial activity against a broad panel of fungi. The central interest by these natural products is related to their bacterial cell division inhibitory effect, and their interaction with FtsZ protein (filamentous temperature-sensitive protein Z), a vital macromolecule for all bacteria. The current project purposes the preparation of two series of compounds. The series I and II will be constitute by dehydrozingerone derivatives substituted with hydroxyl and methoxyl groups at ring B, respectively. Compounds from the series A and B were designed by molecular hybridization, using curcumin and cinnamaldehyde as prototypes. Compounds will be prepared by aldol condensation reactions, under acid catalysis. Compounds will be tested against Gram-positive, Gram-negative bacteria and Mycobacterium species, using sensitive and resistant strains. The toxicity of selected antibacterial compounds will be evaluated on macrophages. The selective index (SI) of compounds will be calculated from IC50 and MBC values, SI = IC50/MBC. IC50 is a half maximal inhibitory concentration to kill macrophages and MBC is a minimum bactericidal concentration. Compounds displayed IS over 10 will be submitted to inhibitory bacterial cell division assays, using FtsZ sedimentation and GTP hydrolysis methods. The chemical data obtained previously by the applicant and the insertion of the project in the Agreement for Scientific and Technological Cooperation between FAPESP and NWO (Netherlands Organisation for Scientific Research)FAPESP 2013/50678-3 corroborate the feasibility and the internationalization proposal.
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