Functional characterization of FIP (FtsH5 interacting protein): a potential regulator of the FtsH5 protease activity in Arabidopsis thaliana

• Grant number: 14/16034-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2014
• End date: July 31, 2019
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Márcio de Castro Silva Filho
• Grantee: Karina Letícia Lopes
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated scholarship(s): 17/15102-4 - Functional characterization of FIP: a putative regulator of FtsH5 in chloroplasts, BE.EP.DR

Abstract

The compartmentalization of eukaryotic cells by endomembrane system enables the separation of chemical reactions and specific metabolic pathways, creating unique environments. Many proteins participate in the establishment and maintenance of these characteristics through a rigorous quality control system. In chloroplasts, the mechanisms of the quality control system eliminate unfolded or photo damaged proteins due to light stress. The PSII reaction center protein D1 participates of the light-driven photosynthetic reactions, and is generally considered to be the main target of photo-oxidative damage which can lead to an inhibition of PSII activity. The FtsH are metalloproteases present as a hetero-hexameric complex anchored in the thylakoid membrane in the chloroplasts of higher plants, their activity is related to the degradation of damaged proteins by oxidation, especially in the repair process of the D1 protein, whose mechanism of regulation remains unclear. In previous works of protein-protein interaction, our group found a plastidial protein interacting with the FtsH complex, called FIP (FtsH5 Interacting Protein). FIP presents a cysteine-rich domain in the C-terminal region was shown to be essential for the interaction with FtsH5 protein. In addition, the FIP transcript levels seems to be regulated by stress conditions, with decreased transcript levels when seedlings were exposed to salt and cold stress, whereas the FtsH5 expression was increased. This study aims to characterize the role of the FIP protein in the activity of the FtsH complex present in the thylakoid membrane of A. thaliana chloroplasts, and in the possibility of FIP as a potential regulator of the FtsH5 protease activity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, KARINA L.; RODRIGUES, RICARDO A. O.; SILVA, MARCOS C.; BRAGA, WILIANE G. S.; SILVA-FILHO, MARCIO C.. The Zinc-Finger Thylakoid-Membrane Protein FIP Is Involved With Abiotic Stress Response in Arabidopsis thaliana. FRONTIERS IN PLANT SCIENCE, v. 9, APR 18 2018. (14/50275-9, 14/16034-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LOPES, Karina Letícia. FIP (FtsH5 Interacting Protein): a zinc-finger protein involved in the abiotic stress response mechanism in Arabidopsis thaliana. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.