Advanced search
Start date
Betweenand

Posttranscriptional regulation of osteoglicyn by miR-155 during myogenesis

Grant number: 14/13783-6
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2014
Effective date (End): February 29, 2016
Field of knowledge:Biological Sciences - Morphology - Histology
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Robson Francisco Carvalho
Grantee:Paula Paccielli Freire Barguil
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Skeletal muscle differentiation, or myogenesis, is a highly orchestrated process in which mononucleated muscle precursor cells, the myoblasts, undergo proliferation. Upon differentiation, they withdraw from the cell cycle, migrate, align with each other, and subsequently fuse to form terminally differentiated multinucleated myotubes. Myogenesis deregulation is related to important skeletal muscle alterations found in muscular dystrophies, cachexia and sarcopenia. Studies reported that the myogenesis is a well-characterized program of cellular differentiation that is highly sensitive to the extracellular matrix. Previous reports have identified the Protein Osteoglycin (OGN, also called mimecan) as one of secreted proteins that showed to be differentially expressed during muscle development. MicroRNAs (miRNAs) are 21-23-nucleotide non-coding RNAs. It has become more evident that this class of small RNAs plays critical roles in the regulation of gene expression at the post-transcriptional level. However, the posttranscriptional regulation of osteoglycin by microRNAs during myogenesis is still completely unknown. Bioinformatics analysis showed that the microRNA mir-155 has complementary pairing with OGN transcript. In this work, we will test the hypothesis that OGN is silencing by miR-155 to regulate the processes of proliferation, migration and differentiation of C2C12 skeletal muscle cells during myogenesis in vitro. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREIRE, PAULA PACCIELLI; FERNANDEZ, GEYSSON JAVIER; CURY, SARAH SANTILONI; DE MORAES, DIOGO; OLIVEIRA, JAKELINE SANTOS; DE OLIVEIRA, GRASIELI; DAL-PAI-SILVA, MAELI; DOS REIS, PATRICIA PINTOR; CARVALHO, ROBSON FRANCISCO. The Pathway to Cancer Cachexia: MicroRNA-Regulated Networks in Muscle Wasting Based on Integrative Meta-Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 8, . (14/13783-6, 12/13961-6)
FREIRE, PAULA PACCIELLI; CURY, SARAH SANTILONI; DE OLIVEIRA, GRASIELI; FERNANDEZ, GEYSSON JAVIER; MORAES, LEONARDO NAZARIO; DA SILVA DURAN, BRUNO OLIVEIRA; FERREIRA, JUAREZ HENRIQUE; FUZIWARA, CESAR SEIGI; KIMURA, EDNA TERUKO; DAL-PAI-SILVA, MAELI; et al. Osteoglycin inhibition by microRNA miR-155 impairs myogenesis. PLoS One, v. 12, n. 11, . (14/13783-6, 12/13961-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BARGUIL, Paula Paccielli Freire. Posttranscriptional regulation of osteoglycin by microRNA miR-155 during myogenesis. 2016. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.