Posttranscriptional regulation of osteoglicyn by miR-155 during myogenesis

• Grant number: 14/13783-6
• Support type: Scholarships in Brazil - Master
• Effective date (Start): October 01, 2014
• Effective date (End): February 29, 2016
• Field of knowledge: Biological Sciences - Morphology
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Robson Francisco Carvalho
• Grantee: Paula Paccielli Freire
• Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Skeletal muscle differentiation, or myogenesis, is a highly orchestrated process in which mononucleated muscle precursor cells, the myoblasts, undergo proliferation. Upon differentiation, they withdraw from the cell cycle, migrate, align with each other, and subsequently fuse to form terminally differentiated multinucleated myotubes. Myogenesis deregulation is related to important skeletal muscle alterations found in muscular dystrophies, cachexia and sarcopenia. Studies reported that the myogenesis is a well-characterized program of cellular differentiation that is highly sensitive to the extracellular matrix. Previous reports have identified the Protein Osteoglycin (OGN, also called mimecan) as one of secreted proteins that showed to be differentially expressed during muscle development. MicroRNAs (miRNAs) are 21-23-nucleotide non-coding RNAs. It has become more evident that this class of small RNAs plays critical roles in the regulation of gene expression at the post-transcriptional level. However, the posttranscriptional regulation of osteoglycin by microRNAs during myogenesis is still completely unknown. Bioinformatics analysis showed that the microRNA mir-155 has complementary pairing with OGN transcript. In this work, we will test the hypothesis that OGN is silencing by miR-155 to regulate the processes of proliferation, migration and differentiation of C2C12 skeletal muscle cells during myogenesis in vitro. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FREIRE, PAULA PACCIELLI; FERNANDEZ, GEYSSON JAVIER; CURY, SARAH SANTILONI; DE MORAES, DIOGO; OLIVEIRA, JAKELINE SANTOS; DE OLIVEIRA, GRASIELI; DAL-PAI-SILVA, MAELI; DOS REIS, PATRICIA PINTOR; CARVALHO, ROBSON FRANCISCO. The Pathway to Cancer Cachexia: MicroRNA-Regulated Networks in Muscle Wasting Based on Integrative Meta-Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 8 APR 2 2019. Web of Science Citations: 1.

FREIRE, PAULA PACCIELLI; CURY, SARAH SANTILONI; DE OLIVEIRA, GRASIELI; FERNANDEZ, GEYSSON JAVIER; MORAES, LEONARDO NAZARIO; DA SILVA DURAN, BRUNO OLIVEIRA; FERREIRA, JUAREZ HENRIQUE; FUZIWARA, CESAR SEIGI; KIMURA, EDNA TERUKO; DAL-PAI-SILVA, MAELI; CARVALHO, ROBSON FRANCISCO. Osteoglycin inhibition by microRNA miR-155 impairs myogenesis. PLoS One, v. 12, n. 11 NOV 21 2017. Web of Science Citations: 2.