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Identification of spring viremia of carps, Koi sleepy disease, carp Pox, Koi herpesvirus and Cyprinid herpesvirus 2 in kois (Cyprinus carpio) and goldfishes (Carassius auratus) from Brazil

Grant number: 14/19180-1
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): December 01, 2014
Effective date (End): November 02, 2015
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering
Principal Investigator:Fabiana Pilarski
Grantee:Pedro Henrique de Oliveira Viadanna
Supervisor abroad: Thomas B. Waltzek
Home Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Local de pesquisa : University of Florida, Gainesville (UF), United States  
Associated to the scholarship:12/16454-8 - Epidemiology and pathology of cyprinids infected with herpesvirus 1, 2 and/or 3, in Brazil, during 2012-2013, BP.DR

Abstract

Carps are the fourth most cultivated species of fish in Brazil, and the species Carassius auratus (goldfish) and Cyprinus carpio (koi carp) are the most important ornamental fishes produced in Brazil, with more than 3 million fishes per year. This production is endangered by infectious diseases. Worldwide, the viruses diseases are the most important for Cyprinids, and the most important are Spring Viremia of Carp (SVC) and koi herpesvirus (CyHV-3). Less importantly, but also a problem in cyprinids farm are: Herpesviral haematopoietic necrosis virus (CyHV-2), carp pox (CyHV-1) and the recently found koi sleepy disease (carp edema virus - CEV). Aim: To diagnose the viral disease: CyHV-1, CyHV-2, CyHV-3, SVC e CEV in koi carps (Cyprinus carpio) and goldfishes (Carassius auratus) from Brazil. The fishes have been collected from the biggest aquaculture farms of koi carps and goldfishes of Brazil. It has been collected 50 specimens of each producer state, and the proportion of koi/goldfish was determined by the amount produced. Total of 207 fishes (105 koi, 102 goldfish) were collected. It is an epidemiological/investigative study. The "n" was determined to have a 90% degree of confidence and an incidence of 45% for each aquaculture farm, and 10% incidence in Brazil. To perform the virus identification, we will use protocols for PCR, according to each virus. (AU)