| Grant number: | 14/02481-9 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Start date: | October 01, 2014 |
| End date: | April 30, 2015 |
| Field of knowledge: | Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture |
| Principal Investigator: | Laura Satiko Okada Nakaghi |
| Grantee: | Sheryll Yohana Corchuelo Chavarro |
| Host Institution: | Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil |
Abstract Gonadotropin releasing hormone (GnRH) is one of the key factors in the neuroendocrine regulation of reproduction in vertebrates. Some fish have three variants of GnRH: GnRH1 involved in the secretion of gonadotropins, GnRH2 that regulates feeding and sexual behaviors and GnRH3 expressed in the olfactory bulb and the nerve terminal nerve, wich innervate the retina and the olfactory epithelium. The zebrafish has two variants of GnRH (GnRH2 and GnRH3), the variant type 3 acts as the GnRH1. It is considered that the function of the GnRH3 in retina is the regulation of visual acuity and retinal processing information. In the olfactory system GnRH regulates the sensitivity in detecting food and improves the intra and interspecific recognition. Some studies show the presence of GnRH receptors on different layers of the retina, however, is still not clear the presence of GnRH receptors in the olfactory epithelium. Therefore the present study aims to assess the effect of olfactory stimuli on the expression of GnRH2, GnRH3 and their receptors in the olfactory-retinal system of the zebrafish Danio rerio. 20 adult females of zebrafish will be exposed to three treatments: (1) water conditioned with adult male zebrafish, (2) water conditioned with immature males of zebrafish and (3) water without stimulation (control). Subsequently, a total RNA extraction from the olfactory epithelium, retina, olfactory bulb, brain and gonads with PureLink ® RNA Mini Kit (Ambion ®) kit will be made. Based on the complete sequence of GnRH2, GnRH3, GnRH-R1, GnRH-R2, R3-GnRH, GnRH-R4, forward and reverse primers will be designed to evaluate the expression genes in different tissues. We expect differences in the gene expression between stimulated and control treatments, specifically we expect an increase in the expression of the GnRH receptors after olfactory stimulation. | |
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