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Role of GnRH and GnRH receptors in the olfactory-retinal system in zebrafish and its relation with sexual preference and reproduction

Grant number: 14/02481-9
Support type:Scholarships in Brazil - Master
Effective date (Start): October 01, 2014
Effective date (End): April 30, 2015
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal researcher:Laura Satiko Okada Nakaghi
Grantee:Sheryll Yohana Corchuelo Chavarro
Home Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Gonadotropin releasing hormone (GnRH) is one of the key factors in the neuroendocrine regulation of reproduction in vertebrates. Some fish have three variants of GnRH: GnRH1 involved in the secretion of gonadotropins, GnRH2 that regulates feeding and sexual behaviors and GnRH3 expressed in the olfactory bulb and the nerve terminal nerve, wich innervate the retina and the olfactory epithelium. The zebrafish has two variants of GnRH (GnRH2 and GnRH3), the variant type 3 acts as the GnRH1. It is considered that the function of the GnRH3 in retina is the regulation of visual acuity and retinal processing information. In the olfactory system GnRH regulates the sensitivity in detecting food and improves the intra and interspecific recognition. Some studies show the presence of GnRH receptors on different layers of the retina, however, is still not clear the presence of GnRH receptors in the olfactory epithelium. Therefore the present study aims to assess the effect of olfactory stimuli on the expression of GnRH2, GnRH3 and their receptors in the olfactory-retinal system of the zebrafish Danio rerio. 20 adult females of zebrafish will be exposed to three treatments: (1) water conditioned with adult male zebrafish, (2) water conditioned with immature males of zebrafish and (3) water without stimulation (control). Subsequently, a total RNA extraction from the olfactory epithelium, retina, olfactory bulb, brain and gonads with PureLink ® RNA Mini Kit (Ambion ®) kit will be made. Based on the complete sequence of GnRH2, GnRH3, GnRH-R1, GnRH-R2, R3-GnRH, GnRH-R4, forward and reverse primers will be designed to evaluate the expression genes in different tissues. We expect differences in the gene expression between stimulated and control treatments, specifically we expect an increase in the expression of the GnRH receptors after olfactory stimulation. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CORCHUELO, SHERYLL; MARTINEZ, EMANUEL R. M.; BUTZGE, ARNO J.; DORETTO, LUCAS B.; RICCI, JULIANA M. B.; VALENTIN, FERNANDA N.; NAKAGHI, LAURA S. O.; SOMOZA, GUSTAVO M.; NOBREGA, RAFAEL H.. Characterization of Gnrh/Gnih elements in the olfacto-retinal system and ovary during zebrafish ovarian maturation. Molecular and Cellular Endocrinology, v. 450, n. C, p. 1-13, . (12/00423-6, 14/02481-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CHAVARRO, Sheryll Yohana Corchuelo. GnRH/GnIH e seus receptores no sistema olfato-retinal de zebrafish. 2015. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

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