Effect of the association between the professional application of TiF4 varnish and daily rinse with a solution containing TiF4 /NaF on dentin erosion in vitro

• Grant number: 14/23472-8
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: January 15, 2015
• End date: February 14, 2015
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Milena Gargaro dos Santos
• Supervisor: Nadine Schlueter
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: Justus Liebig University Giessen (JLU), Germany
• Associated to the scholarship: 12/22642-1 - Effect of the association between the professional application of TiF4 varnish and the daily rinse with TiF4/ NaF solution on enamel erosion in vitro, BP.IC

Abstract

The aim of this study is to investigate the effect of the association between the professional application of TiF4 varnish (one application) and the at-home application of a solution containing TiF4/NaF (daily application) against dentin erosion in vitro. For that, 108 bovine root dentin samples will be polished and protected in 2/3 of the surface using nail varnish. They will be distributed into 6 experimental groups (n=18/group): Group 1 - control (no treatment); Group 2 - rinse solution containing TiF4/NaF (500 ppm F-, pH 4.4); Group 3 - NaF varnish (24500 ppm F-, pH 5.0); Group 4 - TiF4 varnish (24500 ppm F-, pH 1.2); Group 5 - TiF4 varnish + group 2; Group 6 - NaF varnish + group 2. The samples belong to the varnishes` groups will be treated once for 6h, before the pH-cycling. After 6h, the varnishes will be removed. The samples belong to the fluoride solution will be treated daily for 7 days (2x1min/day), after the first and the last erosive challenges. The erosive challenges will be done 4x90s/day, using 0.1% citric acid solution (pH 2.5). Between the erosive challenges, the samples will be immersed in artificial saliva (remineralization). After 7 days, the dentin loss will be measured using contact profilometry (µm). The data will be statistically analyzed (p<0.05). Three samples from each group will be analyzed by scanning electron microscope in the University of Giessen, under supervision of Nadine Schlueter, who is expert in the preparation of the dentin samples in the critical point for getting images in a cross-sectional view. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAGALHAES, ANA CAROLINA; DOS SANTOS, MILENA GARGARO; COMAR, LIVIA PICCHI; RABELO BUZALAF, MARILIA AFONSO; GANSS, CAROLINA; SCHLUETER, NADINE. Effect of a Single Application of TiF4 Varnish versus Daily Use of a Low-Concentrated TiF4/NaF Solution on Tooth Erosion Prevention in vitro. Caries Research, v. 50, n. 5, p. 462-470, 2016. (12/22642-1, 12/20698-0, 14/23472-8)