In a previous study by this research group, the feasibility of biosurfactant production by Bacillus amyloliquefaciens was assessed by solid state fermentation and the use of the biosurfactant produced in the biological treatment of industrial effluents containing fats. Thus, the wheat bran was used as substrate and the fermentation occurred in polyethylene bags with 10 grams of solid substrate plus glycerol or frying oil used as inductors and phosphate buffer solution, cassava or water, used as humidifiers, the concentration to obtain 40, 60 and 80% humidity, incubated at 37°C for 96 hours. Analysis of surface tension, emulsification index and emulsificant activity were made. The best results of biosurfactant production were applied at concentrations of 2, 5 and 8 % in anaerobic reactor to treat wastewater from dairy and meat processing. In this project, we intend to evaluate the variation of the concentration of iron content, manganese, sodium and its various alternatives for production by Bacillus amyloliquefaciens sources. Analyzes of surface tension and emulsification index will be made. Will also evaluate the antimicrobial activity for biosurfactants produced that show the best results.
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