Genome-wide expression and DNA methylation analysis in an antipsychotic-naive first episode of psychosis cohort

• Grant number: 14/22223-4
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): February 23, 2015
• Effective date (End): February 13, 2016
• Field of knowledge: Health Sciences - Medicine - Psychiatry
• Principal researcher: Síntia Iole Nogueira Belangero
• Grantee: Marcos Leite Santoro
• Supervisor abroad: Gerome Breen
• Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Research place: King's College London, England
• Associated to the scholarship: 12/12686-1 - Potential genetic markers in early phases of schizophrenia, BP.DR

Abstract

The investigation of individuals during their first-episode psychosis (FEP), before the progression and the treatment with antipsychotic medications, is helpful for understanding the complexity of schizophrenia. Considering that schizophrenia is a chronic condition, disease progression and use of antipsychotic medication can confound results on gene expression and DNA methylation. Our main aim of this study is to identify genetic markers using transcriptomic and methylomic approaches in a longitudinal cohort of FEP. The patients will be assessed in the baseline, all antipsychotic naïve (anFEP, N=60) and after eight weeks (FEP-8w, N=60) of antipsychotic treatment, beside, 60 Health controls (HC) will be assessed. The DNA Genotyping array will be performed using the PsychChip array. Transcriptomic and methylomic approaches will be performed using the HumanHT-12 v4 Expression BeadChip (approx. 25,000 genes) and the Infinium HumanMethylation450 BeadChip (approx. 490,000 methylation sites), respectively. With these data, we expect to find potential blood biomarkers for disease (anFEPxHC) and for treatment response (anFEP x FEP-8w). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTORO, MARCOS LEITE; OTA, VANESSA; DE JONG, SIMONE; NOTO, CRISTIANO; SPINDOLA, LETICIA M.; TALARICO, FERNANDA; GOUYEA, EDUARDO; LEE, SANG HYUCK; MORETTI, PATRICIA; CURTIS, CHARLES; PATEL, HAMEL; NEWHOUSE, STEPHEN; CARYALHO, CAROLINA MUNIZ; GADELHA, ARY; CORDEIRE, QUIRINO; BRESSAN, RODRIGO AFFONSECA; BELANGERO, SINTIA IOLE; BREEN, GEROME. Polygenic risk score analyses of symptoms and treatment response in an antipsychotic-naive first episode of psychosis cohort. TRANSLATIONAL PSYCHIATRY, v. 8, AUG 31 2018. Web of Science Citations: 6.