Imnunonanosensors based on atomic force microscopy for multiple sclerosis studies

• Grant number: 14/21530-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2015
• End date: December 31, 2017
• Field of knowledge: Biological Sciences - Biology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Fabio de Lima Leite
• Grantee: Pâmela Soto Garcia
• Host Institution: Centro de Ciências e Tecnologias para a Sustentabilidade (CCTS). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil

Abstract

The Multiple Sclerosis (MS) is an inflammatory, autoimmune and demyelinating disease with prevalence in female young adults. Concerning the actual knowledge about the disease, it is known that auto-reactive antibodies act against myelin's proteins and up to now, there is no efficient method to previously diagnose MS. In this context, it is imperative the investigation of an accurate diagnostic method for obtaining precise and early results. The present project intends to use the Atomic Force Microscopy (AFM) to develop sensitive immunonanosensors in order to measure the antigen-antibody chemical interaction mechanisms involved in the MS immunopathogeny. Additionally, by combining molecular modeling techniques and experimental approaches it is expected to achieve the target amino acids sequences direct related to MS. Consequently, it will be possible to measure the energy of that antigen-antibody complex. The uniqueness of this project relies on the use of a technique that focus the immobilization of the antigen-antibody complex on nanosurfaces (AFM apparatus) to detect MS target molecules identification. It is also included the availability to combine the results between the data obtained at the experimental analysis with the ones from computational simulation. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA, PAMELA SOTO; BRUM, DORALINA GUIMARAES; OLIVEIRA JR, OSVALDO N.; HIGA, AKEMI MARTINS; MAGALHAES IERICH, JESSICA CRISTIANE; MORAES, ARIANA DE SOUZA; SHIMIZU, FLAVIO MAKOTO; OKUDA-SHINAGAWA, NANCY M.; PERONI, LUIS ANTONIO; DA GAMA, PAULO DINIZ; et al. Nanoimmunosensor based on atomic force spectroscopy to detect anti-myelin basic protein related to early-stage multiple sclerosis. ULTRAMICROSCOPY, v. 211, APR 2020. (14/50869-6, 12/50839-4, 13/14262-7, 14/12082-4, 14/21530-0, 15/06847-0, 15/14360-4)

HIGA, AKEMI M.; MAMBRINI, GIOVANNI P.; IERICH, JESSICA C. M.; GARCIA, PAMELA S.; SCRAMIN, JULIANA A.; PERONI, LUIS A.; OKUDA-SHINAGAWA, NANCY M.; MACHINI, M. TERESA; TRIVINHO-STRIXINO, FRANCISCO; LEITE, FABIO L.. Peptide-Conjugated Silver Nanoparticle for Autoantibody Recognition. Journal of Nanoscience and Nanotechnology, v. 19, n. 12, p. 7564-7573, DEC 2019. (14/12082-4, 10/10813-0, 14/21530-0, 15/06847-0)

MAGALHAES IERICH, JESSICA CRISTIANE; BRUM, DORALINA GUIMARAES; MORAES, ARIANA DE SOUZA; HIGA, AKEMI MARTINS; GARCIA, PAMELA SOTO; MIYAZAKI, CELINA MASSUMI; FERREIRA, MARYSTELA; PERONI, LUIS ANTONIO; DE OLIVEIRA, GUEDMILLER SOUZA; FRANCA, EDUARDO DE FARIA; et al. Antibody-mediated biorecognition of myelin oligodendrocyte glycoprotein: computational evidence of demyelination-related epitopes. SCIENTIFIC REPORTS, v. 9, FEB 14 2019. (13/09746-5, 14/12082-4, 14/15093-7, 14/21530-0, 12/50839-4, 15/05283-6, 14/26369-3, 14/50869-6, 15/06847-0, 16/19387-0)

OLIVEIRA, GUEDMILLER S.; IERICH, JESSICA C. M.; MORAES, ARIANA S.; SILVA, GISELA B. R. F.; LIU, YANYUN; NETO, LOURIVAL R. DE S.; FARIA, ROBERTO R.; FRANCA, EDUARDO F.; FREITAS, LUIZ C. G.; BRIGGS, JAMES M.; et al. Immobilization and unbinding investigation of the antigen -antibody complex using theoretical and experimental techniques. JOURNAL OF MOLECULAR GRAPHICS & MODELLING, v. 86, p. 219-227, JAN 2019. (12/50839-4, 15/05283-6, 13/09746-5, 14/21530-0, 14/12082-4, 14/26369-3, 15/06847-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GARCIA, Pâmela Soto. Development of atomic force microscopy nanoimmunosensor applied to the survey of multiple sclerosis. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Medicina Tropical de São Paulo (IMT) São Paulo.