Prospection of anti-inflammatory activity in native fruit species from Brazil

• Grant number: 13/26251-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: January 01, 2015
• End date: February 29, 2016
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Pedro Luiz Rosalen
• Grantee: Josy Goldoni Lazarini
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil

Abstract

In the search for alternative sources of new drugs, Brazilian fruit species could be an alternative option to be explored. Flavonoids present in their composition stand out for having anti-inflammatory action. Brazil is considered a major global producer of fruits, and has introduced fruits like açaí to the world market and encouraged research of other species as to their food and therapeutic potential, which add value and constitute a successful model of Brazilian agro-business. The aim of this project is to evaluate the anti-inflammatory activity, mechanism of action and phytochemical profile of fruit species native to Brazil. We selected 5 native fruits, namely: Eugenia leitonii (guava - piranga), Eugenia brasiliensis (grumixama), Eugenia involucrata (cherry Grand River), Eugenia Myrcianthes (ubajaí) and Garcinea brasiliensis (bacupari - mirim) for the exploration of anti-inflammatory activity. The plants will be harvested at pre-determined periods, and their parts (fruits, leaves, seed, bark) will be processed for obtaining hydroethanolic extracts (80:20, v/v), which will be lyophilized and stored at -20°C. The anti-inflammatory activity of extracts will be performed by means of a carrageenan-induced leukocyte migration model. We will select the extract with the best activity and submit it to a dose-response curve, followed by the evaluation of possible pathways of mechanisms of actios using models of Carrageenan-induced paw edema, quantification in vivo of inflammatory mediators (TNF-± ,IL-1², IL-10, CXCL1/KC and LTB4) and quantification in vitro of (TNF-±, IL-1², IL-10 e LTB4) with cells RAW 264.7, assessment of ICAM-1, VCAM-1, P- and E-selectin by Western blot and cell cytotoxicity (RAW 264.7). The chemical evaluation of the active extract will be performed by analysis of phenolic compounds, high performance liquid chromatography on reversed phase (HPLCRF-DAD) and mass spectrometry (HPLC/MS). This project is expected to contribute with information about the anti-inflammatory activity, mechanism of action and phytochemical profile of native species as well as to add value to domestic products. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LAZARINI, JOSY GOLDONI; FRANCHIN, MARCELO; INFANTE, JULIANA; RIZZATO PASCHOAL, JONAS AUGUSTO; FREIRES, IRLAN ALMEIDA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Anti-inflammatory activity and polyphenolic profile of the hydroalcoholic seed extract of Eugenia leitonii, an unexplored Brazilian native fruit. Journal of Functional Foods, v. 26, p. 249-257, OCT 2016. (13/26251-0, 13/13190-2)

ORLANDI SARDI, JANAINA DE CASSIA; FREIRES, IRLAN ALMEIDA; LAZARINI, JOSY GOLDONI; INFANTE, JULIANA; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Unexplored endemic fruit species from Brazil: Antibiofilm properties, insights into mode of action, and systemic toxicity of four Eugenia spp.. Microbial Pathogenesis, v. 105, p. 280-287, APR 2017. (11/15984-0, 13/26251-0)

LAZARINI, JOSY GOLDONI; ORLANDI SARDI, JANAINA DE CASSIA; FRANCHIN, MARCELO; NANI, BRUNO DIAS; FREIRES, IRLAN ALMEIDA; INFANTE, JULIANA; RIZZATO PASCHOAL, JONAS AUGUSTO; DE ALENCAR, SEVERINO MATIAS; ROSALEN, PEDRO LUIZ. Bioprospection of Eugenia brasiliensis, a Brazilian native fruit, as a source of anti-inflammatory and antibiofilm compounds. BIOMEDICINE & PHARMACOTHERAPY, v. 102, p. 132-139, JUN 2018. (13/26251-0, 13/13190-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LAZARINI, Josy Goldoni. Prospection of the anti-inflamatory activity of Eugenia leitonii D. Legrand, a Brazilian native fruit. 2016. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.