| Grant number: | 14/23051-2 |
| Support Opportunities: | Scholarships in Brazil - Master |
| Start date: | March 01, 2015 |
| End date: | September 30, 2015 |
| Field of knowledge: | Biological Sciences - Microbiology - Applied Microbiology |
| Agreement: | Coordination of Improvement of Higher Education Personnel (CAPES) |
| Principal Investigator: | André Ricardo de Lima Damasio |
| Grantee: | Felipe Calzado |
| Host Institution: | Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil |
| Associated research grant: | 12/20549-4 - Secretion of heterologous glycoproteins in Aspergillus: effect of glycosylation pattern in functional parameters of glycosyl hydrolases, AP.BIOEN.JP |
Abstract As a way to reduce the use of energy from fossil fuels and increase the use of renewable energy, the second-generation ethanol obtained from the conversion of lignocellulosic plant biomass into fermentable sugars has been the focus of much research nowadays as a way to enable its use. Filamentous fungi stand out in the production and secretion of lignocellulolytic homologous and heterologous enzymes, but little is known about the mechanisms of protein secretion used by these organisms. Thought about it, the use of the model organism Aspergillus nidulans becomes an important resource for studies to be genetically well characterized. The objective of this master's project is to perform a comparative analysis of the mechanisms of secretion of different heterologous proteins in A. nidulans obtaining the transcriptome data generated from RNA-seq technique (Next Generation RNA sequencing) and analyze data using bioinformatics tools. To monitor the secretion process, we will adopt four target genes: GH43 arabinase (AbnA), GH7 cellobiohydrolase (CbhI), GH3 beta-glucosidase (BglC) and thermophilic GH5 mananase (1542.1). These genes were previously cloned and transformed into A. nidulans, for AbnA and CbhI was reached high levels of secretion, unlike BglC and 1542.1 in which the secretion levels were minimal. With the obtained results, our main goal is at least to demonstrate how the strain adapts to heterologous expression of different target genes, including a gene of thermophilic trait. (AU) | |
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