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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Molecular basis of substrate recognition and specificity revealed in family 12 glycoside hydrolases

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Author(s):
Calzado, Felipe ; Prates, Erica T. ; Goncalves, Thiago A. ; Rubio, Marcelo V. ; Zubieta, Mariane P. ; Squina, Fabio M. ; Skaf, Munir S. ; Damasio, Andre R. L.
Total Authors: 8
Document type: Journal article
Source: Biotechnology and Bioengineering; v. 113, n. 12, p. 2577-2586, DEC 2016.
Web of Science Citations: 4
Abstract

Fungal GH12 enzymes are classified as xyloglucanases when they specifically target xyloglucans, or promiscuous endoglucanases when they exhibit catalytic activity against xyloglucan and -glucan chains. Several structural and functional studies involving GH12 enzymes tried to explain the main patterns of xyloglucan activity, but what really determines xyloglucanase specificity remains elusive. Here, three fungal GH12 enzymes from Aspergillus clavatus (AclaXegA), A. zonatus (AspzoGH12), and A. terreus (AtEglD) were studied to unveil the molecular basis for substrate specificity. Using functional assays, site-directed mutagenesis, and molecular dynamics simulations, we demonstrated that three main regions are responsible for substrate selectivity: (i) the YSG group in loop 1; (ii) the SST group in loop 2; and (iii) loop A3-B3 and neighboring residues. Functional assays and sequence alignment showed that while AclaXegA is specific to xyloglucan, AtEglD cleaves -glucan, and xyloglucan. However, AspzoGH12 was also shown to be promiscuous contrarily to a sequence alignment-based prediction. We find that residues Y111 and R93 in AtEglD harbor the substrate in an adequate orientation for hydrolysis in the catalytic cleft entrance and that residues Y19 in AclaXegA and Y30 in AspzoGH12 partially compensate the absence of the YSG segment, typically found in promiscuous enzymes. The results point out the multiple structural factors underlying the substrate specificity of GH12 enzymes. Biotechnol. Bioeng. 2016;113: 2577-2586. (c) 2016 Wiley Periodicals, Inc. (AU)

FAPESP's process: 14/23051-2 - Comparative analysis of the heterologous protein secretion mechanisms in Aspergillus nidulans
Grantee:Felipe Calzado
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/20549-4 - Secretion of heterologous glycoproteins in Aspergillus: effect of glycosylation pattern in functional parameters of glycosyl hydrolases
Grantee:André Ricardo de Lima Damasio
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
FAPESP's process: 13/08293-7 - CCES - Center for Computational Engineering and Sciences
Grantee:Munir Salomao Skaf
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 13/24988-5 - Secretion of heterologous glycoproteins in Aspergillus: effect of glycosylation pattern in functional parameters of glycosyl hydrolases
Grantee:Marcelo Ventura Rubio
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 14/06923-6 - Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models
Grantee:Andre Luis Ferraz
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants