Effect of different resin based materials on the eroded enamel subjected to erosion and / or abrasion in vitro and in situ

• Grant number: 14/02384-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2015
• End date: May 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Daniela Rios Honório
• Grantee: Gabriela Cristina de Oliveira
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

The resin based materials have been studied as a preventive method to dental erosion showing promising effects in preventing erosion progression. This study aimed to evaluate in vitro and in situ the efficacy of the use of infiltrant, resin sealant and adhesive system on eroded initial enamel for the inhibition of erosive lesions progression subjected to erosion and/or abrasion. This study will be divided into three subprojects, which will have the sample size determined by pilot studies. Subprojects 1 and 2 will be conducted in vitro, the subproject 1 will assess two factors: material application, in 2 levels (with/without removal of material on enamel surface) and type of treatment, in 5 levels (control, infiltrant, sealant, conventional and self-etching adhesive system).On subproject 2, the factors under study will be type of wear, in three levels (erosion, abrasion, erosion+abrasion) and type of treatment in 5 levels (control, infiltrant, sealant, conventional and self-etching adhesive system). The studies will be performed with previously eroded enamel specimens (0.01M HCl, pH 2.3 for 30s), randomized among groups and treated accordance to the manufacturer's recommendations. In subproject 1, on half of the specimens, after the application of the materials, their excess on enamel surface will be removed prior to light curing. In subproject 1, the erosive cycling will be for 5 days (HCl for 30s and artificial saliva for 2h, 4 times/day). In subproject 2, the erosive cycling will be similar to the subproject 1 and there will be also abrasive cycling (2000 cycles of oscillatory movements with the force of 1.5 N) and association of both (HCl for 30s and artificial saliva for 2h, 4 times/day + 2 times/day after the first and last 2000 cycles erosive cycling oscillatory movements with the force of 1.5N). The subproject 3 will be conducted in situ with two factors under study: type of wear in three levels (erosion, abrasion, erosion+abrasion) and type of treatment in 4 levels (control, sealant, adhesive system and infiltrant). Previously eroded enamel blocks (immersion in 0.01 M HCl, pH 2.3 for 30 s), randomized among groups, will be treated in accordance to the manufacturer's recommendations. After treatments, the blocks will be subjected to erosive and/or abrasive cycling during two crossover phases of 30 days with an interval of 15 days between them. At each phase three different materials and two types of wear will be tested (6 blocks of each material, eachtwo blocks will be subjected separately to erosion, abrasion and erosion/abrasion). In all subprojects, the results will be evaluated by profilometry analysis (initial, after treatment and after erosive cycling) and submitted to appropriate statistical analysis, considering the factors under study. The significance level for all tests is 5%. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RIOS, D.; OLIVEIRA, G. C.; ZAMPIERI, C. R.; JORDAO, M. C.; DIONISIO, E. J.; BUZALAF, M. A. R.; WANG, L.; HONORIO, H. M.. Resin-Based Materials Protect Against Erosion/Abrasion-a Prolonged In Situ Study. Operative Dentistry, v. 44, n. 3, p. 302-311, MAY-JUN 2019. (14/02384-3)

GENOVEZ TEREZA, GUIDA PAOLA; DE OLIVEIRA, GABRIELA CRISTINA; DE ANDRADE MOREIRA MACHADO, MARIA APARECIDA; DE OLIVEIRA, THAIS MARCHINE; DA SILVA, THIAGO CRUVINEL; RIOS, DANIELA. Influence of removing excess of resin-based materials applied to eroded enamel on the resistance to erosive challenge. Journal of Dentistry, v. 47, p. 49-54, APR 2016. (14/02384-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Gabriela Cristina de. Effect of resin-based materials application against erosion/abrasion - a in vitro and in situ study. 2017. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.