Activity of paclitaxel carried by carbon nanotubes on prostate cancer cells

• Grant number: 14/26032-9
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2015
• Effective date (End): February 28, 2017
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Ramon Kaneno
• Grantee: Edson José Comparetti
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Prostatic cancer is the main type of tumor in male worldwide, and the frequency of relapsing and metastatic diseases demands the development of new approaches for diagnosis and therapy. Considering this cenario, this project aims to develop nanocarriers to transport antineoplasic agents into tumor cells. For this purpose, carbon nanotubes will be functionalized with polyethylenimina (PEI) for incorporation of paclitaxel, a first line medication for prostate cancer. Then, nanotubos will be recovered with anti-prostate specific membrane antigens (anti-PSMA) to address then for PSMA+ tumor cells. In addition, particles will be conjugated to fluorescein isotiocianate in order to follow the cell adhesion/internalization. Nanocarries will be tested in vitro for: a) specificity for PSMA+ tumor cells; b) uptake by target cells, and c) cytotoxicity of paclitaxel immobilized on nanotubos. Target specificity will be tested by exposition of nanocarrier to LNCap cells (PSMA+ cellprostate cancer), HCT-116 cells (PSMA-colon cancer cells), peripheral blood lymphocytes and monocytes. Uptake of carriers by these targets will be analyzed by imunofluorescence microscopy and flow cytometry. Cytotoxicyty of immobilized paclitaxel will be checked by in vitro exposition of target cells to nanocarriers and further analysis of ability to reduce methyl-tetrazolium salt (MTT). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COMPARETTI, EDSON JOSE; PEDROSA, VALBER DE ALBUQUERQUE; KANENO, RAMON. Carbon Nanotube as a Tool for Fighting Cancer. BIOCONJUGATE CHEMISTRY, v. 29, n. 3, p. 10-pg., 2018-03-01. (14/26032-9)

COMPARETTI, EDSON JOSE; PEDROSA, VALBER DE ALBUQUERQUE; KANENO, RAMON. Carbon Nanotube as a Tool for Fighting Cancer. BIOCONJUGATE CHEMISTRY, v. 29, n. 3, SI, p. 709-718, MAR 2018. (14/26032-9)

COMPARETTI, EDSON JOSE; ROMAGNOLI, GRAZIELA GORETE; GORGULHO, CAROLINA MENDONCA; PEDROSA, VALBER DE ALBUQUERQUE; KANENO, RAMON. Anti-PSMA monoclonal antibody increases the toxicity of paclitaxel carried by carbon nanotubes. Materials Science & Engineering C-Materials for Biological Applications, v. 116, NOV 2020. (12/20494-5, 14/26032-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

COMPARETTI, Edson José. Activity of paclitaxel carried by carbon nanotubes on prostate cancer cells. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.