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Activity of paclitaxel carried by carbon nanotubes on prostate cancer cells

Grant number: 14/26032-9
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2015
Effective date (End): February 28, 2017
Field of knowledge:Biological Sciences - Immunology - Cellular Immunology
Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Ramon Kaneno
Grantee:Edson José Comparetti
Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


Prostatic cancer is the main type of tumor in male worldwide, and the frequency of relapsing and metastatic diseases demands the development of new approaches for diagnosis and therapy. Considering this cenario, this project aims to develop nanocarriers to transport antineoplasic agents into tumor cells. For this purpose, carbon nanotubes will be functionalized with polyethylenimina (PEI) for incorporation of paclitaxel, a first line medication for prostate cancer. Then, nanotubos will be recovered with anti-prostate specific membrane antigens (anti-PSMA) to address then for PSMA+ tumor cells. In addition, particles will be conjugated to fluorescein isotiocianate in order to follow the cell adhesion/internalization. Nanocarries will be tested in vitro for: a) specificity for PSMA+ tumor cells; b) uptake by target cells, and c) cytotoxicity of paclitaxel immobilized on nanotubos. Target specificity will be tested by exposition of nanocarrier to LNCap cells (PSMA+ cellprostate cancer), HCT-116 cells (PSMA-colon cancer cells), peripheral blood lymphocytes and monocytes. Uptake of carriers by these targets will be analyzed by imunofluorescence microscopy and flow cytometry. Cytotoxicyty of immobilized paclitaxel will be checked by in vitro exposition of target cells to nanocarriers and further analysis of ability to reduce methyl-tetrazolium salt (MTT). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COMPARETTI, EDSON JOSE; ROMAGNOLI, GRAZIELA GORETE; GORGULHO, CAROLINA MENDONCA; PEDROSA, VALBER DE ALBUQUERQUE; KANENO, RAMON. Anti-PSMA monoclonal antibody increases the toxicity of paclitaxel carried by carbon nanotubes. Materials Science & Engineering C-Materials for Biological Applications, v. 116, . (12/20494-5, 14/26032-9)
COMPARETTI, EDSON JOSE; PEDROSA, VALBER DE ALBUQUERQUE; KANENO, RAMON. Carbon Nanotube as a Tool for Fighting Cancer. BIOCONJUGATE CHEMISTRY, v. 29, n. 3, SI, p. 709-718, . (14/26032-9)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
COMPARETTI, Edson José. Activity of paclitaxel carried by carbon nanotubes on prostate cancer cells. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.

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