Molecular analysis of pathogenic fungus in bats from the region of Botucatu SP

• Grant number: 14/23234-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2015
• End date: February 28, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Helio Langoni
• Grantee: Giselle Souza da Paz
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Currently, among the emerging diseases zoonoses are the most, and bats play an important role as a source of infection or transmission of infectious agents. Histoplasmosis, cryptococcosis and paracoccidioidomycosis are reported fungal diseases in several animal species, their agents can be isolated from grottos, caves, soil and animal feces. Therefore, the objective of this study is to perform molecular detection of fungi Histoplasma capsulatum, Cryptococcus neoformans and Paracoccidioidis brasiliensis in bats the from Botucatu region and in the state of São Paulo. Lung and liver bats will be collected during the period of 12 months for the research and diagnosis of H. capsulatum, C. neoformans and P. brasiliensis using the techniques of molecular diagnostics: Polymerase Chain Reaction (PCR) and Nested-PCR. Georeferencing of samples with the aid of Arcgis 10.1® software to generate maps of the distribution of points of gathering the animals will be held. The study design is a series of cases, by analyzing the frequency of the collected species, animal habits and found location. Statistical analysis will be performed through the distribution and frequency of features of interest cases such as species, feeding habits of bats and found location. Then subjected to analysis with PROC FREC procedure (SAS Institute, 2011). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA PAZ, GISELLE S.; ADORNO, BRUNNA MAYLA V.; RICHINI-PEREIRA, VIRGINIA B.; BOSCO, SANDRA M. G.; LANGONI, HELIO. Infection by Histoplasma capsulatum, Cryptococcus spp. and Paracoccidioides brasiliensis in bats collected in urban areas. TRANSBOUNDARY AND EMERGING DISEASES, v. 65, n. 6, p. 1797-1805, DEC 2018. (15/07627-4, 14/23234-0)

DA PAZ, GISELLE S.; ADORNO, BRUNNA MAYLA V.; RICHINI-PEREIRA, VIRGINIA B.; BOSCO, SANDRA M. G.; LANGONI, HELIO. Infection by Histoplasma capsulatum, Cryptococcus spp. and Paracoccidioides brasiliensis in bats collected in urban areas. TRANSBOUNDARY AND EMERGING DISEASES, v. 65, n. 6, p. 9-pg., 2018-12-01. (14/23234-0, 15/07627-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PAZ, Giselle Souza da. Research molecular of pathogenic fungi in bats from Botucatu region. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.