Metabolomics analysis of drought-tolerant soybean genotypes using Nuclear Magnetic Resonance

• Grant number: 14/24006-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2015
• End date: February 17, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Luiz Alberto Colnago
• Grantee: Isabel Duarte Coutinho
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated scholarship(s): 15/21388-2 - Unidimensional and multidimensional NMR approaches to covering the plant metabolome, BE.EP.PD

Abstract

Since 2003 the use of metabolomics approaches in scientific research has enabled a breakthrough in the understanding about the function and role of genes in plants. In addition to evaluating the metabolism of plants as a whole, this kind of approach has also allowed the quality control of genetically modified organisms. In this context, the aim of this project is to use high resolution NMR for metabolomics analysis of leaves and roots of genetically modified and non-modified soybean under control and water deficit conditions. Firstly we will assess the use of High Resolution NMR in solution and heterogeneous solid (HR-MAS High Resolution Magic Angle Spin) for hydrogen (1H) and phosphorus (31P) nuclei. After the selection step of the technique, the metabolic fingerprinting of drought-tolerant soybean and non-modified genotypes, both grown in a greenhouse, will be obtained. The data will be analyzed using chemometric tools. Molecular markers will be identified using two-dimensional NMR techniques, as well by building an "in house" databank of LC-DAD-SPE-NMR spectroscopic data. The identification of molecular markers will enable the detection of phenotypic changes at plant metabolic level when subjected to water deficit, and assist in the construction of metabolic networks that are activated in genotypes containing the gene constructs AtAREB1. This approach will lead to better understanding of the molecular, physiological and genetic aspects involved the genetically modified plants.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COUTINHO, ISABEL D.; MORAES, TIAGO BUENO; MERTZ-HENNING, LILIANE MARCIA; NEPOMUCENO, ALEXANDRE LIMA; GIORDANI, WILLIAN; MARCOLINO-GOMES, JULIANA; SANTAGNELI, SILVIA; COLNAGO, LUIZ ALBERTO. Integrating High-Resolution and Solid-State Magic Angle Spinning NMR Spectroscopy and a Transcriptomic Analysis of Soybean Tissues in Response to Water Deficiency. Phytochemical Analysis, v. 28, n. 6, p. 529-540, NOV-DEC 2017. (14/24006-0)