Regulation of mitochondrial function by hypoxia inducible factor (HIF-1) in insulin resistant muscle cells

• Grant number: 15/02278-1
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: July 01, 2015
• End date: November 30, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Metabolism and Bioenergetics
• Principal Investigator: Leonardo dos Reis Silveira
• Grantee: Michel Barbosa de Araújo
• Supervisor: Nathan A. Berger
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Case Western Reserve University, United States
• Associated to the scholarship: 12/17416-2 - REGULATION OF MITOCHONDRIAL FUNCTION AND METABOLISM OF GLUCOSE AND FATTY ACIDS BY FACTOR OF HYPOXIA INDUCIBLE (HIF-1a) MUSCLE CELLS IN INSULIN RESISTANCE, BP.PD

Abstract

Skeletal muscle insulin resistance is a common feature in diabetic population. One target that has been given some attention is the transcriptional factor hypoxia inducible factor (HIF-1±). Our proposal was to evaluate the role of HIF-1± in insulin resistant skeletal muscle cells. The skeletal muscle cells will isolated from quadriceps muscle from Wistar rats and cultured in DMEM (Ethics protocol no. 063/2012 FMRP-USP). After differentiation, the cells will exposed to different nutrients: glucose, palmitic acid 600 ¼M, AICAR (500 ¼M) and cobalt chloride (CoCl2 - 250 ¼M). The oxygen consumption will measured using a Clark-type electrode (Hansatech Instruments). Analyses of western blotting will performed for proteins quantifying HIF-1± and and ²-actin blotting was used as an internal control. In addition, the mRNA level of HIF-1± and ²-actin will determined by qPCR-Realtime. (AU)