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Stress of endoplasmic reticulum and expression of VAPB and Rab1 in cultured motor neurons from a mouse model of Amyotrophic Lateral Sclerosis

Grant number: 15/06786-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2015
Effective date (End): May 31, 2016
Field of knowledge:Biological Sciences - Morphology - Cytology and Cell Biology
Principal researcher:Merari de Fátima Ramires Ferrari
Grantee:Elisangela de Paula Silva
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center, AP.CEPID

Abstract

Endoplasmic reticulum is a multifunctional organelle involved in protein synthesis, processing, and modeling, as well as participates of intracellular traffic and calcium signaling. VAPB is an endoplasmic reticulum transnsmembrane protein that interacts with mitochondria during calcium homeostasis, participates of cellular signalling, cytoarchitecture, unfolded protein response and traffic between endoplasmic reticulum and golgi apparatus. This trafficking is also performed by Rab1. Endoplasmic reticulum stress might be associated to dysfunction of VAPB and Rab1, and it is common to many neurodegenerative diseases such as amyotrophic lateral sclerosis. In view of this, this study aims to investigate the association among the expression of VAPB, Rab1, and endoplasmic reticulum stress in cultured motor neurons of transgenic mice expressing mutant SOD1 (G93A), non-mutant SOD1, or control mice B6SJL. The methodology will employ a genotyping, culture of motor neurons, immunocytochemistry, western blot, and exogenous expression of Rab1.

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