Cloning and expression of a 41-Kilodalton protein from Cryptosporidium parvum in Escherichia coli

• Grant number: 15/07147-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: January 01, 2016
• End date: December 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcelo Vasconcelos Meireles
• Grantee: Milena Sato de Souza
• Supervisor: Mark Christopher Jenkins
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Institution abroad: Beltsville Agricultural Research Center, United States
• Associated to the scholarship: 13/24082-6 - Expression and purification of Cryptosporidium parvum COWP5 recombinant protein aiming the production of polyclonal IgY in chickens for diagnostic purpose, BP.DR

Abstract

Cryptosporidium spp. are intracellular protozoan parasites that infect the epithelial cells of digestive and respiratory tracts of a wide variety of vertebrates. This parasite may cause self-limiting diarrheal illness in immunocompetent individuals, but in immunocompromised patients it may be fatal. Cryptosporidium parvum has many animal hosts and it is one of the dominant Cryptosporidium species infecting humans. The development of diagnosis and control strategies for cryptosporidiosis in humans has become the main challenge of epidemiological studies. Management of sources of the parasite represents the most important means of preventing widespread outbreaks of cryptosporidiosis, and management is dependent on sensitive and specific diagnostic reagents. At present, all detection methods for C. parvum are confounded by cross-reactivity of antisera to bacteria naturally present in environmental samples. Our goal is to express recombinant CP41 by cloning, produce antisera specific for CP41 and teste the antibodies against CP41 for binding to C. parvum oocysts. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, MILENA SATO; O'BRIEN, CELIA; SANTIN, MONICA; JENKINS, MARK. A highly sensitive method for detecting Cryptosporidium parvum oocysts recovered from source and finished water using RT-PCR directed to Cryspovirus RNA. Journal of Microbiological Methods, v. 156, p. 77-80, JAN 2019. (15/07147-2)