Analysis of biomolecules damage from motor neuron cell under neurodegeneration and oxidative conditions

• Grant number: 15/08937-7
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): July 01, 2015
• Effective date (End): June 30, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Principal researcher: Giselle Cerchiaro
• Grantee: Aryane Alves Vigato
• Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil

Abstract

This project will use methodologies for the detection of oxidative stress and neurodegeneration biomarkers in cultured cells, such as detection and quantification of the oxidation of proteins, lipids and DNA.For this purpose, oxidative stress are generated by hydrogen peroxide incubation and neurodegenerative stress by beta-amyloid peptides in cell culture, respectively. The result of the project will integrate the map of cellular biomarkers for the differentiation of these types of stresses, in progress in the laboratory. The student has already started her work in the lab as a voluntary and demonstrated excellent development and maturity.