Remineralization and protective effect of a new calcium silicate/phosphate toothpaste associated with a dual-phase calcium silicate/phosphate gel against enamel abrasion/erosion challenges in vitro

• Grant number: 15/07675-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2015
• End date: May 31, 2016
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Alessandra Buhler Borges
• Grantee: Morgana de Menezes Maia
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Dental erosion is a multifactorial process that involves the tooth demineralization by chemical action of extrinsic or intrinsic acids. Dentifrices containing calcium silicate and sodium phosphate salts and high levels of fluoride are potentially remineralizing and protective agents against demineralization; however their efficiency in the treatment of early erosion and its progression has not been fully established. Thus, it is appropriate to investigate the remineralizing and protective capacity of toothpastes that contain promising substances for controlling dental erosion. The dentifrices tested will be: Coc - 0ppm of fluoride (Bitufo) as a control; PE: potassium nitrate and sodium fluoride (1450ppm-Sensodyne); PAER - Arginine, calcium carbonate and sodium monofluorphosphate (1450ppm - Colgate); Reg - calcium silicate/sodium phosphate (Unilever); and RegGD - calcium silicate/ sodium phosphate associated with a dual phase gel of calcium silicate/sodium phosphate and 1450 ppm of sodium monofluorphosphate (Unilever). Polished bovine enamel specimens will be prepared and subjected to erosive challenge-abrasive for 5 days, with 0.3% citric acid (pH=2.6 -2min - 6x/dia), two daily abrasion cycles (15s - 200g each) and artificial salivare mineralization (1h). The remineralization capacity and protection against initial erosion will be measured by microhardness at the first day of the cycle and the structure loss will be assessed by profilometry at the third and fifth days of the cycle. Data will be submitted to normality and homocedasticity tests and, from them, the appropriate statistical tests of variance and multiple comparison (=5%) will be determined.