Remineralization and protective effect of a new calcium silicate/phosphate toothpaste associated with a dual-phase calcium silicate/phosphate gel against enamel abrasion/erosion challenges in vitro.

• Grant number: 15/07675-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2015
• End date: May 31, 2016
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Alessandra Buhler Borges
• Grantee: Morgana de Menezes Maia
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Dental erosion is a multifactorial process that involves the tooth demineralization bychemical action of extrinsic or intrinsic acids. Dentifrices containing calcium silicateand sodium phosphate salts and high levels of fluoride are potentially remineralizingand protective agents against demineralization; however their efficiency in the treatmentof early erosion and its progression has not been fully established. Thus, it isappropriate to investigate the remineralizing and protective capacity of toothpastes thatcontain promising substances for controlling dental erosion. The dentifrices tested willbe: Coc - 0ppm of fluoride (Bitufo) as a control; PE: potassium nitrate and sodiumfluoride (1450ppm-Sensodyne); PAER - Arginine, calcium carbonate and sodiummonofluorphosphate (1450ppm - Colgate); Reg - calcium silicate/sodium phosphate(Unilever); and RegGD - calcium silicate/ sodium phosphate associated with a dualphase gel of calcium silicate/sodium phosphate and 1450 ppm of sodiummonofluorphosphate (Unilever). Polished bovine enamel specimens will be preparedand subjected to erosive challenge-abrasive for 5 days, with 0.3% citric acid (pH=2.6 -2min - 6x/dia), two daily abrasion cycles (15s - 200g each) and artificial salivaremineralization (1h). The remineralization capacity and protection against initialerosion will be measured by microhardness at the first day of the cycle and the structureloss will be assessed by profilometry at the third and fifth days of the cycle. Data will besubmitted to normality and homocedasticity tests and, from them, the appropriatestatistical tests of variance and multiple comparison (=5%) will be determined.