DNA methylation profile of peripheral blood in attention deficit hyperactivity disorder (ADHD) carriers by array

• Grant number: 15/05350-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2015
• End date: October 31, 2016
• Field of knowledge: Health Sciences - Medicine
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Leslie Domenici Kulikowski
• Grantee: Thais Virginia Moura Machado Costa
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Attention Deficit Hyperactivity Disorder (ADHD) is one of the most common childhood brain disorders and can continue through adolescence and adulthood affects approximately 5.3% of children worldwide. Despite the prevalence and heritability ADHD, biological markers do not exist. Behavioral genetic studies have demonstrated conclusively that genetic influences play a role in the etiology of ADHD. Similarly, environmental factors also play an important role in the etiology of ADHD. DNA methylation is an important epigenetic mechanism associated with transcriptional silencing of genes in CpG islands. Therefore, it is essential to investigate whether the genetic alterations of ADHD are related to the activation or inactivation of genes by DNA methylation. For the study, individuals (n=30) up to 16 years of age with a clinical diagnosis of ADHD and their parents will be recruited in the Unit of Genetics of the Institute of Child, Hospital das Clínicas of the FMUSP (HC-FMUSP). Blood samples will be collected, and the extraction of lymphocyte DNA will be performed. Methylation assays will be carried out by array to verify the methylation status of CpG islands in thousands of genomic regions. In addition, the SNP array in order to better understanding of the findings will be performed. Data will be analyzed using GenomeStudio software and specific packages within the environment R. Finally, the relationships between epigenetic data obtained and the phenotype of individuals with ADHD will be made in order to obtain a DNA methylation profile of peripheral blood in individuals with ADHD. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CARVALHO, GLEYSON FRANCISCO DA SILVA; COSTA, THAIS VIRGINIA MOURA MACHADO; NASCIMENTO, AMOM MENDES; WOLFF, BEATRIZ MARTINS; DAMASCENO, JULIAN GABRIEL; VIEIRA, LUCAS LIRO; ALMEIDA, VANESSA TAVARES; DE OLIVEIRA, YANCA GASPARINI; DE MELLO, CLAUDIA BERLIM; MUSZKAT, MAURO; et al. DNA methylation epi-signature and biological age in attention deficit hyperactivity disorder patients. CLINICAL NEUROLOGY AND NEUROSURGERY, v. 228, p. 7-pg., 2023-04-11. (15/05350-5, 14/02565-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

COSTA, Thais Virginia Moura Machado. DNA methylation profile of individuals with attention deficit hyperactivity disorder (ADHD) performed by array and MLPA techniques. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.