Association oxidative stress with activation of inflammatory response and oxidative metabolism of neutrophils in obese dogs

• Grant number: 15/06467-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2015
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Paulo César Ciarlini
• Grantee: Anelise Maria Bosco
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Obesity has a high incidence and currently is the prinicpal nutritional disease of dogs. Obesity has been associated with oxidative stress and increased risk of tumors, diabetes, pancreatitis, infections, heart disease and joint problems. There is evidence that obesity in humans promotes a generalized inflammatory reaction, changing the neutrophil function and compromising the immunological defense. In dogs, little is known about the relationship between obesity, oxidative stress, inflammatory response and oxidative metabolism of neutrophils. In this sense we will investigate the hypothesis that oxidative stress is associated with the obese dogs inflammation and activation of neutrophil oxidative metabolism. For such a control group (n = 25) consisted of healthy dogs with good body condition is compared with an obese group (n = 25) and overweight (n = 25). Of all selected dogs will be measured changes in markers of obesity (body score, body mass index, adiponectin and leptin plasma); oxidative stress markers (albumin, bilirubin, uric acid, GGT, total antioxidant capacity, total concentration of oxidant and plasma MDA); markers of inflammatory (C-reactive protein, TNF., IFN-gamma, IL-6 and IL-10) and neutrophil production of reactive oxygen species (superoxide and hydrogen peroxide). Expected to gain a better understanding of the mechanisms of such changes that could guide new strategies treatments for control of the deleterious effects associated with canine obesity. This proposal is linked to the research project recently approved by FAPESP (2014 / 20662-0) and will help the candidate Doctoral Scholarship will build on its line of research on oxidative stress started the Masters with support from FAPESP (2011 / 16018-0) (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BOSCO, A. M.; ALMEIDA, B. F. M.; VALADARES, T. C.; BAPTISTIOLLI, L.; HOFFMANN, D. J.; PEREIRA, A. A. F.; LIMA, V. M. F.; CIARLINI, P. C.. Preactivation of neutrophils and systemic oxidative stress in dogs with hyperleptinemia. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, v. 202, p. 18-24, AUG 2018. (15/06467-3, 14/20662-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BOSCO, Anelise Maria. Association of obesity with the activation of neutrophil oxidative metabolism in dogs. 2017. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba Araçatuba.