Application of different enzyme engineering strategies to obtain derivatives of new immobilized-stabilized lipases

• Grant number: 15/10530-2
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: November 01, 2015
• End date: October 31, 2016
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Cristiane Sanchez Farinas
• Grantee: Erick de Abreu Silveira
• Supervisor: Jose M. Guisan Seijas
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Institution abroad: Instituto de Catálisis y Petroleoquímica (ICP), Spain
• Associated to the scholarship: 13/20826-0 - Production of lipase by the combined fermentation process and its application in the modification of oils and fats, BP.DR

Abstract

Lipases (triacylglycerol hydrolase, EC 3.1.1.3) are enzymes of great industrial interest, because they are able to catalyze triacylglycerides hydrolysis, esterification and transesterification reactions. Aiming to find potential producers of lipase, in the initial stage of this work it was carried out a screening of filamentous fungi that were grown in plates and subsequent confirmation by solid state fermentation (SSF) using wheat bran and olive oil as inducer. Next, the strain A. niger C was selected for lipase production experiments, making use of agro-industrial waste from the palm oil processing industry as substrates for solid state fermentation (SSF) and submerged fermentation (SmF ). However, for the efficient application of lipases, challenges related to their stability in the reaction medium and to the reuse of the biocatalyst need to be investigated. In this context, it is of great importance the knowledge of the technologies proposed at this doctorate internship at the Departamento de Biocatalisis of the Instituto de Catálisis y Petroleoquímica, Madrid, Spain, involving the synthesis of stabilized derivatives of new lipases by different enzyme engineering strategies, including the immobilization process in hydrophobic media and post-production modification of lipases through glycosylation, pegylation and hydroxylation. Finally, the novel derivatives of lipases will be evaluated in transesterification reactions for the production of biodiesel and synthesis of pharmaceutical and cosmetic bioproducts. It should behighlight the valuable "know-how" to be achieved abroad and applied in Brazil to improve the research in the biochemistry laboratory of UFSCar and Embrapa.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVEIRA, ERICK ABREU; MORENO-PEREZ, SONIA; BASSO, ALESSANDRA; SERBAN, SIMONA; PESTANA-MAMEDE, RITA; TARDIOLI, PAULO W.; SANCHEZ-FARINAS, CRISTIANE; CASTEJON, NATALIA; FERNANDEZ-LORENTE, GLORIA; ROCHA-MARTIN, JAVIER; et al. Biocatalyst engineering of Thermomyces Lanuginosus lipase adsorbed on hydrophobic supports: Modulation of enzyme properties for ethanolysis of oil in solvent-free systems. Journal of Biotechnology, v. 289, p. 126-134, JAN 10 2019. (16/10636-8, 15/10530-2, 13/20826-0)