Detection of changes related to breast cancer using charge sensors or mass sensors coupled to DNA monolayers

• Grant number: 15/14403-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2015
• End date: January 31, 2016
• Field of knowledge: Engineering - Electrical Engineering - Electrical, Magnetic and Electronic Measurements, Instrumentation
• Principal Investigator: Marcelo Mulato
• Grantee: Marina Ribeiro Batistuti Sawazaki
• Supervisor: Pedro Miguel de Lemos Correia Estrela
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: University of Bath, England
• Associated to the scholarship: 13/26133-7 - Detection of changes related with breast cancer using charge sensors or mass sensors coupled to DNA monolayers, BP.DR

Abstract

Identification of cancer-specific markers at an early stage can greatly improve clinical success rate and reduce cancer-related mortality. Faster, more sensitive and robust biosensors are being developed in a wide range of techniques for the detection of disease markers, but very few devices enter clinical trials for cancer diagnosis.This collaboration focuses on the development of biosensor arrays that enable the simultaneous detection of a panel of biomarkers specific to breast cancer (BC) or prostate cancer (PCa). The proposal is built on recent developments by thesupervisors and their collaborators, namely by integrating a range of developed electrochemical, semiconductor-based, and plasmonic biosensors suitable for in vitro cancer diagnostics. Novel biosensor devices will also be developed to the detectionof panels of microRNAs, which can target cancer-associated mRNAs transcribed by tumour suppressors and oncogenes. In Brazil, some electrochemical parameters have been developed but now the student requires further and more in depth training. At the University of Bath (UBath) the development of novel biosensor devices for the simultaneous detection of panels of microRNAs will be conducted, using signal probe molecules and nanoparticles for signal amplification and microRNAs detection to lead to personalized fingerprints of cancer onset and progression. At the University of the West of England (UWE) the molecular biology aspects of microRNAs will be developed. Most of the initial sensing development work at UBath will be on DNA analogues, but the transition to actual microRNA samples requires the expertise of the group at UWE

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARYA, SUNIL K.; ZHURAUSKI, PAVEL; JOLLY, PAWAN; BATISTUTI, MARINA R.; MULATO, MARCELO; ESTRELA, PEDRO. Capacitive aptasensor based on interdigitated electrode for breast cancer detection in undiluted human serum. BIOSENSORS & BIOELECTRONICS, v. 102, p. 106-112, APR 15 2018. (15/14403-5)

JOLLY, PAWAN; BATISTUTI, MARINA R.; MIODEK, ANNA; ZHURAUSKI, PAVEL; MULATO, MARCELO; LINDSAY, MARK A.; ESTRELA, PEDRO. Highly sensitive dual mode electrochemical platform for microRNA detection. SCIENTIFIC REPORTS, v. 6, NOV 8 2016. (15/14403-5)

BATISTUTI, MARINA R.; BUENO, PAULO R.; MULATO, MARCELO. The importance of the assembling of DNA strands on the performance of electrochemical genosensors. Microchemical Journal, v. 159, DEC 2020. (17/24839-0, 15/14403-5, 14/09562-4)