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Dynamics of functional genes associated to nitrogen and carbon changes in T-FACE system

Grant number: 15/10985-0
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2015
Effective date (End): July 31, 2017
Field of knowledge:Agronomical Sciences - Agronomy - Soil Science
Principal Investigator:Tsai Siu Mui
Grantee:Tayná Negri Kühn
Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


Although the concentration of carbon dioxide (CO2) is unlikely to have direct effects on the microbial communities of the soil, the increase of atmospheric CO2 can affect soil ecosystems indirectly through the plant responses. This study will be conducted to test the hypothesis that exposure of plants to elevated CO2 concentrations and temperature rise could impact the soil microbes responsible for key processes in carbon (C) and nitrogen (N) cycling. The controlled experiment was conducted in the experimental field of the University of São Paulo, Ribeirão Preto - SP. The plants Stylosanthes capitata Vogel and Panicum maximum L. subgenus were grown in a consortium under T-FACE (free-air temperature controlled enhancement carbon dioxide free-air enrichment) in ambient concentration (CO2 and temperature) and high concentration (600 ppm CO2 and temperature + 2 ° C than the room) distributed in the following treatments: control (C); with temperature and CO2 concentration environment, high CO2 (eC); with concentration of CO2 to 600 ppm, high TºC (eT); with the elevated temperature at + 2 °C to room temperature and the combination of the effects and T + eC (Et + eC). This type of study has been scarce since demand technology and infrastructure (control of temperature effects and greater concentration of CO2) require a more robust financial support which is not feasible should it not have the financial assistance under the climate change of FAPESP process 2008 / 58075-8. Thus, soils were collected at a depth of 0-10 cm for chemical and physical molecular analysis. In this study will be used the PCR quantitative technique (qPCR - quantitative Polymerase Chain Reaction) to quantify the functional genes of the total microbial community (16S rRNA and rpoB) methane oxidating community (pmoA), methane producing (mcrA), fixing community nitrogen (nifH) and denitrifying (nosZ) from soil. (AU)

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