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Digestive enzymes activity of intestine of Piaractus mesopotamicus juveniles fed different levels of DDGS

Grant number: 15/21245-7
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): November 01, 2015
Effective date (End): February 29, 2016
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
Principal researcher:Elisabete Maria Macedo Viegas
Grantee:Katia Rodrigues Batista de Oliveira
Supervisor abroad: Maria Helena Tabuaco Rego Martins Peres
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Research place: Universidade do Porto (UP), Portugal  
Associated to the scholarship:14/16685-5 - Corn distiller's dried grains with soluble in diets for pacu juveniles Piaractus mesopamicus (Holmberg 1987), BP.MS


To evaluate the effects of corn DDGS inclusion and soybean meal replacement on digestive enzymes of Piaractus mesopotamucus intestine, fish with 21 grams of mean weight were stocked in 20 cubic aquariums (130L) at density of 15 fish aquarium-1 and feeding test diets for 100 days. Due to compound the treatments were formulated five diets with equal protein and energy content and increasing DDGS levels (0, 10, 20, 30 e 40%). Each aquarium was considered as an experimental unity, in a completely randomized design (five treatments and four replicates). At the end of the trial, all fish were euthanized, weighted and gutted. Intestines from three fish per aquarium were collected and divided in three segments: anterior, medium and posterior. Samples were frozen in liquid nitrogen and stored at -80°C to future analysis. The activity of total protease, ± amylase, lipase, trypsin and chymotrypsin will be determinate. Results will be expressed in U/mg of protein and data will be submitted to one-way ANOVA statistical analyses. Significant differences will be evaluated by Linear Regression at 5% of probability. After getting results will be possible obtain a better understanding about corn DDGS metabolism by fish organism, through correlation of digestive enzymes activities and fish growth performance. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BATISTA OLIVEIRA, KATIA RODRIGUES; PERES, HELENA; OLIVA-TELES, AIRES; MARCONI, JOANA NIZE; PAULINO, RENAN ROSA; DIOGENES, ALEXANDRE FIRMINO; MACEDO VIEGAS, ELISABETE MARIA. Maize distillers dried grains with solubles alter dietary digestibility and improve intestine health of pacu, Piaractus mesopotamicus juveniles. BRITISH JOURNAL OF NUTRITION, v. 125, n. 12, p. 1331-1343, . (14/16685-5, 15/21245-7)

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