Extraction and chemical identification of natural colorants produce by Penicillium purpurogenum DPUA 1275

• Grant number: 15/04751-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2015
• End date: August 31, 2018
• Field of knowledge: Engineering - Chemical Engineering
• Principal Investigator: Valéria de Carvalho Santos Ebinuma
• Grantee: Fábio Aurélio Esteves Torres
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 14/01580-3 - Biotechnological process for the development of new natural colorants from microorganisms for industrial application, AP.JP

Abstract

In last year's there is a trend to replace synthetic colorants by natural ones because they have lower adverse effects to human health, may possess biological activity, and are well accepted by consumers. These compounds can be produced by plants, insects and microorganisms; however, microorganisms have gained importance due to bioprocess that can generate high throughput. In this scenario, the filamentous fungi Penicillium purpurogenum, is not mycotoxigenic and is an alternative source of natural colorants with antioxidant and antimicrobial activity. Thereby, efficient techniques for the extraction and/or purification of these biocompounds from the fermented broth are of great interest aiming its industrial application. The objective of this project is to purify natural colorants from fermented broth of P. purpurogenum DPUA 1275 by liquid-liquid extraction with organic solvents, followed by determination of their chemical structure and evaluate aqueous two-phase systems based on Ionic Liquids (ABS-IL) as an alternative extraction technique. Initially the conventional technique liquid-liquid extraction using organic solvents of different polarities will be used to purify the natural colorants and subsequently, determinate its chemical structure by spectroscopic methods. In the second stage of this work, ABS-IL formed by different families of ionic liquids will be studied, followed by countercurrent chromatography to increase the purity of the extracted colorant. These techniques will be compared in order to show the effectiveness of the proposed alternative system. Thus, this project will address the extraction and/or purification of natural colorants by conventional and alternative techniques, which will be the basis for the extraction and/or purification of other compounds from microrganism. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ESTEVES TORRES, FABIO AURELIO; DE ALMEIDA FRANCISCO, ANA CLARA; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA DE CARVALHO. Imidazolium-based ionic liquids as co-surfactants in aqueous micellar two-phase systems composed of nonionic surfactants and their aptitude for recovery of natural colorants from fermented broth. Separation and Purification Technology, v. 196, n. SI, p. 262-269, MAY 8 2018. (16/01076-9, 14/01580-3, 15/04751-6)

ESTEVES TORRES, FABIO AURELIO; DE ALMEIDA FRANCISCO, ANA CLARA; BRANDAO PEREIRA, JORGE FERNANDO; SANTOS-EBINUMA, VALERIA DE CARVALHO. Imidazolium-based ionic liquids as co-surfactants in aqueous micellar two-phase systems composed of nonionic surfactants and their aptitude for recovery of natural colorants from fermented broth. Separation and Purification Technology, v. 196, p. 8-pg., 2018-05-08. (15/04751-6, 14/01580-3, 16/01076-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

TORRES, Fábio Aurélio Esteves. Extração e identificação química de colorantes naturais produzidos por Talaromyces amestolkiae. 2018. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.