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THP-1 culturing cells standardization to evaluate miRNAs and their target genes response against influence of anti-inflammatory stimuli

Grant number: 15/21184-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2015
Effective date (End): November 30, 2016
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Maria de Fatima Sonati
Grantee:Roberta Dorta Ferreira
Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:14/00984-3 - Red blood cell disorders: pathophysiology and new therapeutic approaches, AP.TEM

Abstract

Cell culturing has become an indispensable tool for distinct areas of knowledge, particularly in the field of molecular genetics. THP-1 cells consist in a human monocytic lineage which might be a good model for in vitro studies, and its differentiation would clarify some mechanisms of gene regulation, physiological functions and some other biological aspects of the macrophage, as defense and secretory mechanisms. This study, therefore, aims to standardize THP-1 culturing cells and anti-inflammatory drugs in culture, in order to assist the evaluation of miRNAs response, involved in the inflammatory response of sickle cell patients with leg ulcers.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MOTA, NATALIA O.; KIMURA, ELZA M.; FERREIRA, ROBERTA D.; PEDROSO, GISELE A.; ALBUQUERQUE, DULCINEIA M.; RIBEIRO, DANIELA M.; SANTOS, MAGNUN N. N.; BITTAR, CRISTINA M.; COSTA, FERNANDO F.; SONATI, MARIA DE FATIMA. Rare alpha(0)-thalassemia deletions detected by MLPA in five unrelated Brazilian patients. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 4, p. 768-773, OCT-DEC 2017. Web of Science Citations: 2.
NATÁLIA O. MOTA; ELZA M. KIMURA; ROBERTA D. FERREIRA; GISELE A. PEDROSO; DULCINÉIA M. ALBUQUERQUE; DANIELA M. RIBEIRO; MAGNUN N. N. SANTOS; CRISTINA M. BITTAR; FERNANDO F. COSTA; MARIA DE FATIMA SONATI. Rare α0-thalassemia deletions detected by MLPA in five unrelated Brazilian patients. GENETICS AND MOLECULAR BIOLOGY, v. 40, n. 4, p. -, Dez. 2017.

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