Characterization of depressive behaviors and morphological aspects involved in early life stress and audiogenic kindling in war strain

• Grant number: 14/17959-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2015
• End date: January 31, 2019
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Norberto Garcia Cairasco
• Grantee: Lívea Dornela Godoy
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 17/11339-0 - Early life stress and neurodevelopment: Contributions of glucocorticoid plasticity to maturational timing., BE.EP.DR

Abstract

Epilepsy is a chronic neurological condition characterized by an enduring predisposition to generate epileptic seizures and by the neurobiological, cognitive, psychological and social consequences of this condition. Depression is a disorder that affects millions of people worldwide, causing a great impact on quality of life of patients. It is well known that depression is a very common psychiatric comorbidity in patients with epilepsy. Data suggest that epilepsy, particularly Temporal Lobe Epilepsy, Depression and other psychiatric disorders such as anxiety possible share pathogenic mechanisms. Accordingly, abnormalities were found in WAR rat that makes this strain an interesting model for the study of stress, epilepsy and neuropsychiatric comorbidities involved. The animals of this strain show higher levels of anxiety, reduction of serotonin in the dorsal raphe nucleus and in addition adrenal hyperplasia. Based on this, this study aims to assess whether the WAR strain presents depressive like behaviors and morphological features associated with the Early Life Stress (ELS) and audiogenic kindling. It will be used an ELS multimodal protocol (four different stressors) PN1 - PN21 in WAR and Wistar rats. Later, from PN70 - 80 it will be induced audiogenic kindling and depressive like behavior evaluated through forced swim test and sucrose consumption. At the end of the behavioral tests it will be performed radioimmunoassay test for quantification of corticosterone levels, immunohistochemical labeling for tryptophan hydroxylase enzyme, doublecortin (DCX) and GAD -67 enzyme. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GODOY, LIVEA DORNELA; GARCIA-CAIRASCO, NORBERTO. Maternal behavior and the neonatal HPA axis in the Wistar Audiogenic Rat (WAR) strain: Early-life implications for a genetic animal model in epilepsy. Epilepsy & Behavior, v. 117, APR 2021. (17/11339-0, 14/17959-1)

CAMPOS-CARDOSO, RODRIGO; GODOY, LIVEA DORNELA; LAZARINI-LOPES, WILLIAN; NOVAES, LEONARDO SANTANA; DOS SANTOS, NILTON BARRETO; PERFETTI, JULIANO GENARO; GARCIA-CAIRASCO, NORBERTO; MUNHOZ, CAROLINA DEMARCHI; PADOVAN, CLAUDIA MARIA. Exploring the light/dark box test: Protocols and implications for neuroscience research. JOURNAL OF NEUROSCIENCE METHODS, v. 384, p. 10-pg., 2023-01-15. (14/17959-1, 14/26753-8, 17/11339-0)

GODOY, LIVEA DORNELA; UMEOKA, EDUARDO H. L.; RIBEIRO, DEIDIANE ELISA; SANTOS, VICTOR RODRIGUES; ANTUNES-RODRIGUES, JOSE; LOURENCO JOCA, SAMIA REGIANE; GARCIA-CAIRASCO, NORBERTO. Multimodal early-life stress induces biological changes associated to psychopathologies. Hormones and Behavior, v. 100, p. 69-80, APR 2018. (13/01737-7, 17/11339-0, 15/18773-1, 14/17959-1)

GODOY, LIVEA DORNELA; ROSSIGNOLI, MATHEUS TEIXEIRA; DELFINO-PEREIRA, POLIANNA; GARCIA-CAIRASCO, NORBERTO; DE LIMA UMEOKA, EDUARDO HENRIQUE. A Comprehensive Overview on Stress Neurobiology: Basic Concepts and Clinical Implications. FRONTIERS IN BEHAVIORAL NEUROSCIENCE, v. 12, JUL 3 2018. (17/11339-0, 14/17959-1, 16/21369-0, 15/18773-1)

CAMPOS-CARDOSO, RODRIGO; NOVAES, LEONARDO SANTANA; GODOY, LIVEA DORNELA; DOS SANTOS, NILTON BARRETO; PERFETTO, JULIANO GENARO; LAZARINI-LOPES, WILLIAN; GARCIA-CAIRASCO, NORBERTO; PADOVAN, CLAUDIA MARIA; MUNHOZ, CAROLINA DEMARCHI. The resilience of adolescent male rats to acute stress-induced delayed anxiety is age-related and glucocorticoid release-dependent. Neuropharmacology, v. 226, p. 9-pg., 2023-01-16. (14/17959-1, 16/03572-3, 14/26753-8, 17/11339-0, 12/24002-0, 18/19599-3, 19/00908-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GODOY, Lívea Dornela. Characterization of behavioral and physiological changes associated with early life stress in experimental models of epilepsy and psychiatric comorbidities. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.