Development of immature forms of Aedes (Stegomyia) aegypti in artificial containers with different salinities

• Grant number: 15/19762-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): January 01, 2016
• Effective date (End): December 31, 2016
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Tamara Nunes de Lima Camara
• Grantee: Rubens Carvalho Silveira
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Aedes aegypti is a mosquito found in urban and suburban areas, showing a endophilic and anthropophilic behavior. The breeding sites of Ae. aegypti are frequently artificial containers such as tires, cans, glasses, bottles, vases, dishes and water tanks.Silva et al 2006 reported the presence of Ae. aegypti in different buildings, such as homes, vacant lots and schools as well as in artificial containers such as drum, tank and barrel. In addition to presenting this adaptation to different breeding sites, Ae. aegypti also plays an important role as arboviruses vector, like dengue, which is endemic in approximately 112 countries, with nearly 100 million cases recorded annually; Chikungunya, which first cases were reported in Brazil in September 2014, and Zika, which first cases were reported in May of 2015. The objective of this study is to investigate the development of immature stages of Ae. aegypti in artificial containers with different salinities. To evaluate the development of the immature stages and the hatching rate, identical four trays will be used, with 1000 ml capacity. The four trays will be filled with 500 ml of water, three with different amounts of free iodine salt (NaCl), which is the main component of marine waters. The fourth tray will contain only distilled water. 100 eggs of Ae. aegypti and 0.20g of Tetramin® fish food wil be placed in each tray. In one of the trays, distilled water will be added to the salt concentration of 35mg / L, while the second tray will have water with salt concentration of 15mg / L, and the third tray will contain the lowest concentration of salt (5mg/L). The fourth tray will contain only distilled water and will serve as a control group. To analyze the eggs hatching rate (proportion of successes) we will apply multinomial analysis and to analyze the average time in each stage will be used an analysis of variance, we will apply ANOVA with a fixed factor. This study will allow a better understanding of the development of Ae. aegypti in containers with saline water in order to better direct monitoring and control strategies for this important measures species. (AU)