Phenotypic analysis, genome and transcriptome sequencing of Campylobacter coli strains isolated from different sources during 16 years in Brazil

• Grant number: 15/23408-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2016
• End date: February 29, 2020
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Juliana Pfrimer Falcão
• Grantee: Carolina Nogueira Gomes
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 18/26043-1 - Whole Genome Sequencing and Comparative Genomics of Campylobacter coli strains isolated from different sources over a 16 year-period in Brazil, BE.EP.DR

Abstract

Campylobacteriosis is a zoonosis of worldwide distribution with significant repercussions on the level of public health and with a high socioeconomic impact. C. coli and C. jejuni species are common causes of gastroenteritis in humans affecting annually approximately 2.4 million people in the United States and an estimated of 9 million people in Europe. However, in Brazil the study and isolation of C. coli are not frequent, making it difficult to evaluate the importance of this pathogen in this country. The aims of this project are to comparatively analyze, by phenotypic tests, sequencing of the genome and transcriptome, 50 C. coli strains isolated from humans, animals, the environment and food during 1995-2011 in Brazil. It will be performed for all strains phenotypic tests of temperature fluctuation, resistance to NaCl, acidic and oxidative stress, invasion of epithelial cells, and proliferation and survival in human and chickens macrophages.It will be done the whole genome sequencing (WGS) of 12 strains of C. coli and the transcriptome sequencing (RNA seq) of two strains of C. coli. For the RNA seq assay two strains of clinical and non-clinical origin will be chosen based on significant differences in the phenotypic tests cited above. The RNAs of these strains will be extracted after tolerance to acid stress and invasion of Caco-2 cells assays. Moreover, the median lethal dose (LD50) of two strains of C. coli will be performed. Due to the paucity of studies involving the C. coli species, studies that aim to elucidate the mechanisms of pathogenicity and virulence are unpublished and of great importance. The results to be obtained should contribute for the better characterization of C. coli isolated during 16 years in Brazil, contributing for a better understanding of this pathogen considered of great importance worldwide.

Scientific publications

(5)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

GOMES, CAROLINA N.; CAMPIONI, FABIO; VILELA, FELIPE P.; DUQUE, SHEILA S.; FALCAO, JULIANA P.. Campylobacter coli strains from Brazil can invade phagocytic and epithelial cells and induce IL-8 secretion. Brazilian Journal of Microbiology, v. 52, n. 2, p. 859-867, JUN 2021. (15/23408-0, 16/24716-3)

GOMES, CAROLINA N.; PASSAGLIA, JAQUELINE; VILELA, FELIPE P.; PEREIRA DA SILVA, FATIMA M. H. S.; DUQUE, SHEILA S.; FALCAO, JULIANA P.. High survival rates of Campylobacter coli under different stress conditions suggest that more rigorous food control measures might be needed in Brazil. FOOD MICROBIOLOGY, v. 73, p. 327-333, AUG 2018. (15/23408-0, 16/24716-3)

GOMES, CAROLINA NOGUEIRA; BARKER, DILLON OLIVER REESE; DUQUE, SHEILA DA SILVA; CHE, EMILY VICTORIA; JAYAMANNA, VASENA; TABOADA, EDUARDO NAPOLEON; FALCAO, JULIANA PFRIMER. Campylobacter coli isolated in Brazil typed by core genome Multilocus Sequence Typing shows high genomic diversity in a global context. INFECTION GENETICS AND EVOLUTION, v. 95, NOV 2021. (18/26043-1, 16/24716-3, 15/23408-0, 19/19338-8)

GOMES, CAROLINA NOGUEIRA; CAMPIONI, FABIO; BARKER, DILLON OLIVER REESE; CHE, EMILY VICTORIA; DUQUE, SHEILA DA SILVA; TABOADA, EDUARDO NAPOLEON; FALCAO, JULIANA PFRIMER. Antimicrobial resistance genotypes and phenotypes of Campylobacter coli isolated from different sources over a 16-year period in Brazil. JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, v. 33, p. 5-pg., 2023-06-01. (22/07013-0, 18/26043-1, 16/24716-3, 19/19338-8, 15/23408-0)

GOMES, CAROLINA NOGUEIRA; FRAZAO, MILIANE RODRIGUES; SERIBELLI, AMANDA APARECIDA; BARKER, DILLON OLIVER REESE; CHE, EMILY VICTORIA; NOGUEIRA, MARA CORREA LELLES; TABOADA, EDUARDO NAPOLEON; FALCAO, JULIANA PFRIMER. Insights on the genomic diversity, virulence and resistance profile of a Campylobacter jejuni strain isolated from a hospitalized patient in Brazil. Brazilian Journal of Microbiology, v. 55, n. 2, p. 11-pg., 2024-03-28. (19/19338-8, 15/23408-0, 18/26043-1, 22/07013-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GOMES, Carolina Nogueira. Phenotypic analysis and whole genome sequencing of Campylobacter coli strains isolated from different sources over 16 year-period in Brazil. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.