Development of Microbial Fuel Biocells for Bioproduction and Oxidation of Ethanol

• Grant number: 15/22973-6
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2016
• Effective date (End): February 28, 2021
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal researcher: Frank Nelson Crespilho
• Grantee: Graziela Cristina Sedenho
• Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated scholarship(s): 17/15714-0 - Redox flow battery coupled with microbial biofuel cell, BE.EP.DR

Abstract

Microbial fuel cells (MFCs) are bioelectrochemical devices that convert chemical energy into electrical energy from organic matter oxidation (fuel), utilizing microorganisms in bioanodes. Thus, this PhD project has the objective to study a biofuel cell using facultative anaerobic microorganisms (fungi specie Saccharomyces cerevisiae) for ethanol bioproduction, in which can be used as a substrate in other systems aimed at obtaining cooperative bioelectrodes in the presence of enzymes. For this, two conversion energy routes will be study: 1) release of reducing agents in solution; 2) electron transfer intracell membrane. In relation of the state-of-art for these systems, the biggest challenge is to develop robust bioelectrodes, with high electrochemical stability and high power density. Furthermore, the literature has few reports about electron transfer intracell membrane kinetics. Thus, principal objective of this project is overcome these challenges, proposing the utilization of bioanodes with modified flexible carbon fibers. Also, it is intended to study the mechanisms involving electron mediators and the bioelectrodes, as well as the comprehension how these microorganisms can be influence kinetic reactions. Lastly, it is intended to optimize MFCs prototypes in laboratory scale to obtain better operating conditions.

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SEDENHO, GRAZIELA C.; MODENEZ, IAGO; MENDES, GIOVANA R.; CRESPILHO, FRANK N. The role of extracellular polymeric substance matrix on Saccharomyces cerevisiae bioelectricity. Electrochimica Acta, v. 393, OCT 10 2021. Web of Science Citations: 0.

MACEDO, LUCYANO J. A.; SANTO, ANDERSON A. E.; SEDENHO, GRAZIELA C.; HASSAN, AYAZ; IOST, RODRIGO M.; FELICIANO, GUSTAVO T.; CRESPILHO, FRANK N. Three-dimensional catalysis and the efficient bioelectrocatalysis beyond surface chemistry. JOURNAL OF CATALYSIS, v. 401, p. 200-205, SEP 2021. Web of Science Citations: 0.

SANCHES, NATALIA M.; HASSAN, AYAZ; MATTIOLI, ISABELA A.; MACEDO, LUCYANO J. A.; SEDENHO, GRAZIELA C.; CRESPILHO, FRANK N. Tuning Vertical Electron Transfer on Graphene Bilayer Electrochemical Devices. ADVANCED MATERIALS INTERFACES, v. 8, n. 20 SEP 2021. Web of Science Citations: 0.

SEDENHO, GRAZIELA C.; HASSAN, AYAZ; MACEDO, LUCYANO J. A.; CRESPILHO, FRANK N. Stabilization of bilirubin oxidase in a biogel matrix for high-performance gas diffusion electrodes. Journal of Power Sources, v. 482, JAN 15 2021. Web of Science Citations: 1.

DE SOUZA, JOAO C. P.; MACEDO, LUCYANO J. A.; HASSAN, AYAZ; SEDENHO, GRAZIELA C.; MODENEZ, IAGO A.; CRESPILHO, FRANK N. In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems. CHEMELECTROCHEM, v. 8, n. 3 DEC 2020. Web of Science Citations: 0.

SEDENHO, GRAZIELA C.; DE PORCELLINIS, DIANA; JING, YAN; KERR, EMILY; MEJIA-MENDOZA, LUIS MARTIN; VAZQUEZ-MAYAGOITIA, ALVARO; ASPURU-GUZIK, ALAN; GORDON, ROY G.; CRESPILHO, FRANK N.; AZIZ, MICHAEL J. Effect of Molecular Structure of Quinones and Carbon Electrode Surfaces on the Interfacial Electron Transfer Process. ACS APPLIED ENERGY MATERIALS, v. 3, n. 2, p. 1933-1943, FEB 2020. Web of Science Citations: 0.

MACEDO, LUCYANO J. A.; HASSAN, AYAZ; SEDENHO, GRAZIELA C.; CRESPILHO, FRANK N. Assessing electron transfer reactions and catalysis in multicopper oxidases with operando X-ray absorption spectroscopy. NATURE COMMUNICATIONS, v. 11, n. 1 JAN 16 2020. Web of Science Citations: 0.

MELO, ANTONIO F. A. A.; SEDENHO, GRAZIELA C.; OSICA, IZABELA; ARIGA, KATSUHIKO; CRESPILHO, FRANK N. Electrochemical Behavior of Cytochrome C Immobilized in a Magnetically Induced Mesoporous Framework. CHEMELECTROCHEM, v. 6, n. 23, p. 5802-5809, DEC 2 2019. Web of Science Citations: 0.

CRESPILHO, FRANK N.; SEDENHO, GRAZIELA C.; DE PORCELLINIS, DIANA; KERR, EMILY; GRANADOS-FOCIL, SERGIO; GORDON, ROY G.; AZIZ, MICHAEL J. Non-corrosive, low-toxicity gel-based microbattery from organic and organometallic molecules. JOURNAL OF MATERIALS CHEMISTRY A, v. 7, n. 43, p. 24784-24787, NOV 21 2019. Web of Science Citations: 0.

ALI, MIAN A.; HASSAN, AYAZ; SEDENHO, GRAZIELA C.; GONCALVES, RENATO V.; CARDOSO, DANIEL R.; CRESPILHO, FRANK N. Operando Electron Paramagnetic Resonance for Elucidating the Electron Transfer Mechanism of Coenzymes. Journal of Physical Chemistry C, v. 123, n. 26, p. 16058-16064, JUL 4 2019. Web of Science Citations: 0.

PAGNONCELLI, KAMILA C.; PEREIRA, ANDRESSA R.; SEDENHO, GRAZIELA C.; BERTAGLIA, THIAGO; CRESPILHO, FRANK N. Ethanol generation, oxidation and energy production in a cooperative bioelectrochemical system. BIOELECTROCHEMISTRY, v. 122, p. 11-25, AUG 2018. Web of Science Citations: 0.

PEREIRA, ANDRESSA R.; SEDENHO, GRAZIELA C.; DE SOUZA, JOAO C. P.; CRESPILHO, FRANK N. Advances in enzyme bioelectrochemistry. Anais da Academia Brasileira de Ciências, v. 90, n. 1, 1, p. 825-857, 2018. Web of Science Citations: 2.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SEDENHO, Graziela Cristina. Bioelectrochemical and bioinspired energy conversion systems: from heterogeneous and extracellular electron transfer to applications in aqueous batteries and biofuel cells. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Química de São Carlos (IQSC/BT) São Carlos.