Fungal biomass of residual bioprocess as nutrient for the cultivation of Burkholderia, Lactobacillus casei, Bifidobacterium, Saccharomyces boulardii, Fusarium, and Trichoderma harzianum

• Grant number: 15/24188-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2016
• End date: January 31, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Valéria Marta Gomes de Lima
• Grantee: Felipe Bertolini Oliveira
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil

Abstract

The microorganisms become important tools in the research in the field of biotechnology. However, many bioprocesses become unviable due to high costs and environmental impacts. Lasiodiplodia theobromae is a filamentous fungus, plant pathogenic widely distributed in Brazil, which produces exopolysaccharide (EPS), relevant product to our research group. In previous studies it was shown that the residual biomass of L. theobromae cultivation has nutritional value and has been successfully used as a supplement in crops for the production of EPS by the fungus itself. This work aims the use of residual biomass L. theobromae as a component of culture medium of some microorganisms of industrial interest: the probiotic Lactobacillus casei, Bifidobacterium and Saccharomyces boulardii, and lipolytic Burkholderia strains can, Fusarium sp and Trichoderma harzianum , targeting both the growth and the production of metabolites. Initially, the fungus L. theobromae preserved at 4 ° C is inoculated into minimal medium VOGEL (1956), glucose (10 g / l) and agar (20g / L) and maintained at 28 ° C, 7 days. The growth medium for the inoculum is pre-Vogel compound and glucose (0.5 g / L), and subsequently transferred to medium composed of Vogel and sucrose (50 g / L), both at pH 5.8, orbital shaking 180 rpm and incubating for 48 h and 72 h, respectively. The biomass is separated by centrifugation (3800 rpm, 35 min), washed with NaCl (0.15 mol / L), autoclaved (1 atm, 15 min), frozen, lyophilized, ground and sieved (0.5 to 1 mm) . The culture medium will be formulated with biomass (10, 20 or 40 g / L) and distilled water where a different microorganism is inoculated, incubated and shaken at a given growth condition and appropriate time. In the design will be used ninhydrin method for determining ±-amino Nitrogen, Bradford for quantification of proteins, phenol-sulfuric acid for measuring the total reducing sugars and sugar hydrolysis of p-palmitate nitrophenyl (pNPP) to determine lipase activity. Cell growth will take place by cell count in Neubauer chamber and also by colony count in plating Pour Plate. It is hoped in this way, check the feasibility of using biomass L. theobromae be used as a component of culture medium, low cost, to obtain biotechnological products with high added value.