PRL and HSPB1 effects in the autophagy and oxidative stress of beta-cells

• Grant number: 16/02881-2
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: May 16, 2016
• End date: December 15, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Leticia Labriola
• Grantee: Letícia Ferreira Terra
• Supervisor: Stephan Lortz
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Hannover Medical School, Germany
• Associated to the scholarship: 13/13914-0 - Autophagy in beta-cells and the cytoprotective roles of prolactin, BP.PD

Abstract

Recent results from our lab have shown that in beta-cells, prolactin (PRL) leads to the upregulation of HSPB1, and that silencing its expression completely abolishes PRL's effect on cytoprotection. Moreover, by performing immunopreciptation flowed by mass spectrometry we now know that only when beta-cells were co-treated with cytokines and PRL, HSPB1 interacts with Superoxide Dismutase 1 and 2, as well as with peroxiredoxin 1 and 3. We have also been analysing the role of PRL in beta-cell autophagy with interesting results. Therefore it would be interesting to study the effects of PRL and the consequent HSPB1 induction on a possible cross-talk between autophagy and oxidative stress in beta-cells. In order to pursue this objective it would be important to investigate the differences in oxidative stress resistance between beta-cells expressing or not HSPB1 submitted to cytokine or rapamycin treatment in the presence or absence of PRL. The results obtained with this project would allow us to propose new hypothesis on the molecular mechanisms involved in beta-cell cytoprotection as well as new therapeutical alternative approaches aiming at increasing beta-cell viability and finally the success of islet transplantation in type 1 diabetes mellitus patients.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TERRA, LETICIA F.; LOBBA, ALINE R. M.. High-fat diet and fish oil affect adipocyte metabolism in a depot-specific manner. JOURNAL OF PHYSIOLOGY-LONDON, v. 595, n. 6, p. 2-pg., 2017-03-15. (13/13914-0, 16/02881-2)

TERRA, LETICIA F.; WAILEMANN, ROSANGELA A. M.; DOS SANTOS, ANCELY F.; GOMES, VINICIUS M.; SILVA, RAILMARA P.; LAPORTE, ANNA; MEOTTI, FLAVIA C.; TERRA, WALTER R.; PALMISANO, GIUSEPPE; LORTZ, STEPHAN; et al. Heat shock protein B1 is a key mediator of prolactin-induced beta-cell cytoprotection against oxidative stress. Free Radical Biology and Medicine, v. 134, p. 394-405, APR 2019. (16/02881-2, 14/17974-0, 13/07029-4, 17/03618-6, 14/02745-6, 13/13914-0)