Molecular characterization and epidemiologic profile of human adenovirus infection in pediatric patients with acute respiratory infection in São Paulo

• Grant number: 15/25643-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: March 01, 2016
• End date: November 30, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Edison Luiz Durigon
• Grantee: Flávio da Silva Mesquita
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Acute respiratory infection (ARI) in the lower respiratory tract is the fourth leading cause of death worldwide. In children under 2 years, Human Adenovirus (HAdV) is responsible for 5 to 15% of viruses responsible for ARIs and 1 to 5% of all respiratory infections, proving the importance of survaillence and monitoring of HAdVs. HAdV belongs to the Adenoviridae Family, Mastadenovirus genus, with a double stranded DNA genome. Its capsomere is formed by 252 units, which 240 are from the hexon protein that forms the faces of the icosahedron. The penton base and fiber proteins form together the vertices of the virus. The HAdV is an important etiologic agent responsible for several patologies in adults and infants, mainly respiratory tract infections, eye infections, gastroenteritis and hemorrhagic cystitis. Nowadays there are 68 known types of HAdV divided into seven species (HAdV-A to HAdV-G). In the case of acute respiratory infections, the HAdV-associated are B, C and E species. The taxonomic characterization of HAdVs is done by sequencing and analysis of hexon (neutralization) and fiber (hemagglutination) genes. Samples collected in the Universitary Hospital in 2015 will be extracted by automated method followed by real time PCR (qPCR). For further genotyping, traditional PCR will be performed with specific primers for the HAdV hexon gene in the samples positive for HAdV in qPCR. Samples visible in agarose gel will be sequenced by SANGER method and then analyzed.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DI PAOLA, NICHOLAS; MESQUITA, FLAVIO S.; LEAL DE OLIVEIRA, DANIELLE BRUNA; VILLABONA-ARENAS, CHRISTIAN JULIAN; POUR, SHAHAB ZAKI; DE SOUSA-CAPRA, CARLA; LOPES, GABRIELA PESSANHA; FERRAZ SANTANA, RUBIA ANITA; REBELLO PINHO, JOAO RENATO; BALARINI, KARIME; et al. An Outbreak of Human Parvovirus B19 Hidden by Dengue Fever. Clinical Infectious Diseases, v. 68, n. 5, p. 810-817, MAR 1 2019. (14/17766-9, 15/25643-7, 13/22136-1)

OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; MENDES, ERICA A.; LADNER, JASON T.; ANDREATA-SANTOS, ROBERT; ARAUJO, DANIELLE B.; BOTOSSO, VIVIANE F.; PAOLA, NICHOLAS D.; NETO, DANIEL F. L.; CUNHA, MARIELTON P.; et al. Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System. Viruses-Basel, v. 10, n. 11, NOV 2018. (13/07467-1, 14/17766-9, 14/27228-4, 14/16333-1, 16/08727-5, 15/25643-7, 14/03911-7, 16/08204-2, 16/20045-7, 13/26942-2, 16/03605-9)