Development of new methodologies using micro-CT for evaluation of physicochemical properties of repair materials and endodontic sealers

• Grant number: 16/00321-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2016
• End date: March 31, 2020
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Mario Tanomaru Filho
• Grantee: Fernanda Ferrari Esteves Torres Carvalho
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 17/22481-1 - Effect of scanning and analysis using micro-CT on evaluation of the properties of repair cements, BE.EP.DR

Abstract

Endodontic materials should have physicochemical properties in accordance with ISO and ADA. Computed microtomography (Micro-CT) is a reproducible and precise technique. During the Master´s degree study, methods were developed for the analysis of material properties. At this stage, further analyzes are being proposed to define the ideal parameters for each property in studies using Micro-CT. The project is divided into subprojects. Subproject 1: aims to evaluate different models, from the proposal of the Master, to study the method of volumetric change evaluation for endodontic materials using Micro-CT. Specimens will be made of acrylic resin models with different dimensions for comparative analysis. The tested sealers will be AHPlus, Sealapex, Fill Canal, MTA, Biodentine and IRM. The scans will be performed after the setting of materials and at 7 and 30 days after immersion in distilled water. Subproject 2: aims to assess the volumetric change in Micro-CT using models according to Carvalho-Junior et al., 2007, idealized for conventional solubility test. The materials will be the same of subproject 1. The specimens will be scanned after setting and immersion in water for 7 and 30 days to obtain the volumetric change and comparison between different dimensions of the specimens. Subproject 3: aims to define flow analysis protocol from the proposal idealized during the Master, using Micro-CT. The materials of subprojects 1 and 2 will be assessed by the conventional test according to standard ISO 6876/2002. A glass device with a central cavity and four grooves in horizontal and vertical directions will be made to flow analysis in Micro CT. The flow of materials and its filling capacity of the central cavity and the side cavities will be evaluated. Subproject 4: The objective of this study will be a correlation between the volumetric change testing and solubility for calcium silicate biomaterials. BC Sealer, MTA Fillapex, Neo MTA and AHPlus will be used. After definitions of the best size of specimens to evaluate the volumetric change, solubility test (ISO 6876/2002) will be realized for correlation with volumetric testing. Subproject 5: aims to assess volumetric change in Micro-CT with images obtained at different resolutions. After definition of the best specimes size to evaluate the volumetric change, the scans will be performed using different resolutions: 5, 9 and 18 ¼m. A comparative analysis to define the best resolution, and analysis software using Micro-CT will be performed for evaluation of suggested properties. For all subprojects, data will be statistically analyzed using ANOVA and Tukey tests, with a significance level of 5%. (AU)

Scientific publications

(18)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINTO, JADER CAMILO; TORRES, FERNANDA FERRARI ESTEVES; LUCAS-OLIVEIRA, EVERTON; BONAGAMBA, TITO JOSE; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. Evaluation of curved root canals filled with a new bioceramic sealer: A microcomputed tomographic study using images with different voxel sizes and segmentation methods. MICROSCOPY RESEARCH AND TECHNIQUE, JUN 2021. (17/19049-0, 18/19665-6, 16/00321-0)

JÁDER CAMILO PINTO; FERNANDA FERRARI ESTEVES TORRES; MARIANA MENA BARRETO PIVOTO-JOÃO; JONI AUGUSTO CIRELLI; JULIANE MARIA GUERREIRO-TANOMARU; MÁRIO TANOMARU-FILHO. Filling Ability and Flow of Root Canal Sealers: A Micro-Computed Tomographic Study. Brazilian Dental Journal, v. 31, n. 5, p. 499-504, 2020-11-02. (17/19049-0, 16/00321-0, 18/19665-6)

FERNANDA FERRARI ESTEVES TORRES; JULIANE MARIA GUERREIRO-TANOMARU; JADER CAMILO PINTO; IDOMEO BONETTI-FILHO; MÁRIO TANOMARU-FILHO. Avaliação do escoamento e preenchimento de cimentos obturadores utilizando diferentes metodologias. Rev. odontol. UNESP, v. 48, 2020-02-10. (17/19049-0, 16/00321-0)

TANOMARU-FILHO, MARIO; ESTEVES TORRES, FERNANDA FERRARI; BOSSO-MARTELO, ROBERTA; CHAVEZ-ANDRADE, GISSELLE MORAIMA; BONETTI-FILHO, IDOMEO; GUERREIRO-TANOMARU, JULIANE MARIA. A Novel Model for Evaluating the Flow of Endodontic Materials Using Micro-computed Tomography. JOURNAL OF ENDODONTICS, v. 43, n. 5, p. 796-800, MAY 2017. (16/00321-0, 15/03437-6, 14/16510-0)

ESTEVES TORRES, FERNANDA FERRARI; BOSSO-MARTELO, ROBERTA; ESPIR, CAMILA GALLETTI; CIRELLI, JONI AUGUSTO; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. Evaluation of physicochemical properties of root-end filling materials using conventional and Micro-CT tests. Journal of Applied Oral Science, v. 25, n. 4, p. 374-380, JUL-AUG 2017. (14/16510-0, 16/00321-0, 15/03437-6)

PINTO, JADER CAMILO; TORRES, FERNANDA FERRARI ESTEVES; LUCAS-OLIVEIRA, EVERTON; BONAGAMBA, TITO JOSE; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. Evaluation of curved root canals filled with a new bioceramic sealer: A microcomputed tomographic study using images with different voxel sizes and segmentation methods. MICROSCOPY RESEARCH AND TECHNIQUE, v. 84, n. 12, p. 2960-2967, DEC 2021. (16/00321-0, 18/19665-6, 17/19049-0)

FERNANDA FERRARI ESTEVES TORRES; JULIANE MARIA GUERREIRO-TANOMARU; ROBERTA BOSSO-MARTELO; CAMILA GALLETTI ESPIR; JÁDER CAMILO PINTO; MÁRIO TANOMARU-FILHO. Micro-computed Tomography Analysis of the Effect of Immersion Time on Volumetric Stability of Different Endodontic Materials. MATERIALS RESEARCH-IBERO-AMERICAN JOURNAL OF MATERIALS, v. 23, n. 4, 2020-08-17. (17/19049-0, 16/00321-0, 18/19665-6)

SANTOS-JUNIOR, AIRTON OLIVEIRA; TANOMARU-FILHO, MARIO; PINTO, JADER CAMILO; MEDINA CARITA TAVARES, KARINA INES; ESTEVES TORRES, FERNANDA FERRARI; GUERREIRO-TANOMARU, JULIANE MARIA. Effect of obturation technique using a new bioceramic sealer on the presence of voids in flattened root canals. BRAZILIAN ORAL RESEARCH, v. 35, p. 9-pg., 2021-01-01. (17/14305-9, 19/22885-0, 17/19049-0, 16/00321-0)

TORRES, F. F. E.; ZORDAN-BRONZEL, C. L.; GUERREIRO-TANOMARU, J. M.; CHAVEZ-ANDRADE, G. M.; PINTO, J. C.; TANOMARU-FILHO, M.. Effect of immersion in distilled water or phosphate-buffered saline on the solubility, volumetric change and presence of voids within new calcium silicate-based root canal sealers. International Endodontic Journal, v. 53, n. 3, p. 7-pg., 2019-10-18. (17/14305-9, 17/19049-0, 16/00321-0)

AIRTON OLIVEIRA SANTOS-JUNIOR; MÁRIO TANOMARU-FILHO; JÁDER CAMILO PINTO; KARINA INES MEDINA CARITA TAVARES; FERNANDA FERRARI ESTEVES TORRES; JULIANE MARIA GUERREIRO-TANOMARU. Effect of obturation technique using a new bioceramic sealer on the presence of voids in flattened root canals. Brazilian Oral Research, v. 35, 2021-02-12. (17/19049-0, 19/22885-0, 16/00321-0, 17/14305-9)

TORRES, F. F. E.; ZORDAN-BRONZEL, C. L.; GUERREIRO-TANOMARU, J. M.; CHAVEZ-ANDRADE, G. M.; PINTO, J. C.; TANOMARU-FILHO, M.. Effect of immersion in distilled water or phosphate-buffered saline on the solubility, volumetric change and presence of voids within new calcium silicate-based root canal sealers. International Endodontic Journal, OCT 2019. (17/14305-9, 16/00321-0, 17/19049-0)

ZORDAN-BRONZEL, CRISTIANE LOPES; ESTEVES TORRES, FERNANDA FERRARI; TANOMARU-FILHO, MARIO; CHAVEZ-ANDRADE, GISSELLE MORAIMA; BOSSO-MARTELO, ROBERTA; GUERREIRO-TANOMARU, JULIANE MARIA. Evaluation of Physicochemical Properties of a New Calcium Silicate-based Sealer, Bio-C Sealer. JOURNAL OF ENDODONTICS, v. 45, n. 10, p. 1248-1252, OCT 2019. (17/14305-9, 16/00321-0, 17/19049-0)

FERNANDA FERRARI ESTEVES TORRES; JULIANE MARIA GUERREIRO-TANOMARU; ROBERTA BOSSO-MARTELO; GISSELLE MORAIMA CHAVEZ-ANDRADE; MARIO TANOMARU FILHO. Solubility, porosity and fluid uptake of calcium silicate-based cements. Journal of Applied Oral Science, v. 26, 2018-05-21. (14/16510-0, 16/00321-0, 15/03437-6)

TANOMARU-FILHO, MARIO; ESTEVES TORRES, FERNANDA FERRARI; CHAVEZ-ANDRADE, GISSELLE MORAIMA; DE ALMEIDA, MADELISE; NAVARRO, LUCIANA GUILHERME; STEIER, LIVIU; GUERREIRO-TANOMARU, JULIANE MARIA. Physicochemical Properties and Volumetric Change of Silicone/Bioactive Glass and Calcium Silicate-based Endodontic Sealers. JOURNAL OF ENDODONTICS, v. 43, n. 12, p. 2097-2101, DEC 2017. (16/00321-0, 15/03437-6)

QUEIROZ, MARCELA B.; TORRES, FERNANDA F. E.; RODRIGUES, ELISANDRA M.; VIOLA, KENNIA S.; BOSSO-MARTELO, ROBERTA; CHAVEZ-ANDRADE, GISSELLE M.; SOUZA, MARINA T.; ZANOTTO, EDGAR D.; GUERREIRO-TANOMARU, JULIANE M.; TANOMARU-FILHO, MARIO. Development and evaluation of reparative tricalciumsilicate-ZrO2-Biosilicatecomposites. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 109, n. 4, p. 468-476, APR 2021. (15/03437-6, 16/11036-4, 16/00321-0, 17/19049-0)

TORRES, FERNANDA FERRRARI ESTEVES; JACOBS, REINHILDE; EZELDEEN, MOSTAFA; GUERREIRO-TANOMARU, JULIANE MARIA; DOS SANTOS, BERNARDO CAMARGO; LUCAS-OLIVEIRA, EVERTON; BONAGAMBA, TITO JOSE; TANOMARU-FILHO, MARIO. Micro-computed tomography high resolution evaluation of dimensional and morphological changes of 3 root-end filling materials in simulated physiological conditions. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 31, n. 2, JAN 21 2020. (17/22481-1, 16/00321-0, 17/19049-0)

QUEIROZ, MARCELA BORSATTO; ESTEVES TORRES, FERNANDA FERRARI; RODRIGUES, ELISANDRA MARCIA; VIOLA, KENNIA SCAPIN; BOSSO-MARTELO, ROBERTA; CHAVEZ-ANDRADE, GISSELLE MORAIMA; GUERREIRO-TANOMARU, JULIANE MARIA; TANOMARU-FILHO, MARIO. Physicochemical, biological, and antibacterial evaluation of tricalcium silicate-based reparative cements with different radiopacifiers. Dental Materials, v. 37, n. 2, p. 311-320, FEB 2021. (16/00321-0, 17/19049-0, 16/11036-4, 17/14305-9)

TORRES, FERNANDA FERRARI ESTEVES; JACOBS, REINHILDE; EZELDEEN, MOSTAFA; FARIA-VASCONCELOS, KARLA DE; GUERREIRO-TANOMARU, JULIANE MARIA; SANTOS, BERNARDO CAMARGO DOS; TANOMARU-FILHO, MARIO. How image-processing parameters can influence the assessment of dental materials using micro-CT. IMAGING SCIENCE IN DENTISTRY, v. 50, n. 2, p. 161-168, JUN 2020. (17/22481-1, 17/19049-0, 16/00321-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARVALHO, Fernanda Ferrari Esteves Torres. Development of new methodologies using Micro-CT to evaluate physicochemical properties of reparative materials and root canal sealers. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.