Antibacterial effect and biocompatibility evaluation of glow discharge plasma treatment on titanium surface: in vitro and in situ study

• Grant number: 15/23118-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2016
• End date: August 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Valentim Adelino Ricardo Barão
• Grantee: João Gabriel Silva Souza
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated scholarship(s): 17/21289-0 - Streptococcus-Candida interactions in biofilms formed on titanium surface, BE.EP.DR

Abstract

The titanium surface treatment with glow-discharge plasma has been used for surface cleaning, reduction of corrosion and changes on the adjacent bone tissue. However, its antibacterial effect, as well as its biocompatibility with gingival tissue cells, has not been previously evaluated, especially considering the abutments. Therefore, the objective of this study is to evaluate the antibacterial effect and biocompatibility of glow-discharge plasma treatment on titanium surface, using in vitro and in situ models. Commercially pure titanium discs (cpTi) will have their surface characterized and subjected to the following treatments: negative control group (use of cpTi discs without surface treatment); and experimental group (cpTi discs with surface treatment by glow-discharge plasma. Three experiments will be conducted. 1) in vitro, blind study, for assessing the biofilm adhesion and formation on cpTi discs. The formation of biofilm (7 days) will be performed considering as inoculum saliva of volunteers. 2) in situ, double blind study, where the volunteers will use during 7 days a palatal appliance containing 4 discs of cpTi (2 treated with control group and 2 experimental group), covered by a plastic mesh to allow the accumulation of biofilm. The biofilm formed will be collected for analysis of total amount of microorganisms via Real Time PCR and characterization of biofilm using confocal laser microscope and scanning electron microscope (Experiment 1 and 2). 3) in vitro study of the culture of fibroblast cells on cpTi discs. After an incubation period, the cell viability and morphology, gene and collagen expression will be evaluated. Groups will be statistically compared using appropriate tests. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUZA, JOAO G. S.; BERTOLINI, MARTINNA; COSTA, RAPHAEL C.; CORDEIRO, JAIRO M.; NAGAY, BRUNA E.; DE ALMEIDA, AMANDA B.; RETAMAL-VALDES, BELEN; NOCITI, FRANCISCO H.; FERES, MAGDA; RANGEL, ELIDIANE C.; et al. Targeting Pathogenic Biofilms: Newly Developed Superhydrophobic Coating Favors a Host-Compatible Microbial Profile on the Titanium Surface. ACS APPLIED MATERIALS & INTERFACES, v. 12, n. 9, p. 10118-10129, MAR 4 2020. (15/23118-2, 16/11470-6)

SOUZA, JOAO G. S.; COSTA OLIVEIRA, BARBARA E.; BERTOLINI, MARTINNA; LIMA, CAROLINA VELOSO; RETAMAL-VALDES, BELEN; DE FAVERI, MARCELO; FERES, MAGDA; BARAO, VALENTIM A. R.. Titanium particles and ions favor dysbiosis in oral biofilms. JOURNAL OF PERIODONTAL RESEARCH, v. 55, n. 2, NOV 2019. (15/23118-2)

SILVA SOUZA, JOAO GABRIEL; BELINE, THAMARA; MATOS, ADAIAS OLIVEIRA; COSTA OLIVEIRA, BARBARA EMANOELE; RICOMINI-FILHO, ANTONIO PEDRO; RICARDO BARAO, VALENTIM ADELINO. Electrochemical behavior of titanium exposed to a biofilm supplemented with different sucrose concentrations. JOURNAL OF PROSTHETIC DENTISTRY, v. 120, n. 2, p. 290-298, AUG 2018. (15/23118-2, 16/11470-6)

SILVA SOUZA, JOAO GABRIEL; LIMA, CAROLINA VELOSO; COSTA OLIVEIRA, BARBARA EMANOELE; RICOMINI-FILHO, ANTONIO PEDRO; FAVERI, MARCELO; SUKOTJO, CORTINO; FERES, MAGDA; DEL BEL CURY, ALTAIR ANTONINHA; RICARDO BARAO, VALENTIM ADELINO. Dose-response effect of chlorhexidine on a multispecies oral biofilm formed on pure titanium and on a titanium-zirconium alloy. BIOFOULING, v. 34, n. 10, p. 1175-1184, 2018. (15/23118-2)

SOUZA, JOAO G. S.; CURY, JAIME A.; RICOMINI FILHO, ANTONIO P.; FERES, MAGDA; DE FAVERI, MARCELO; BARAO, VALENTIM A. R.. Effect of sucrose on biofilm formed in situ on titanium material. Journal of Periodontology, v. 90, n. 2, p. 141-148, FEB 2019. (15/23118-2)

SOUZA, JOAO G. S.; CORDEIROL, JAIRO M.; LIMA, V, CAROLINA; BARAO, VALENTIM A. R.. Citric acid reduces oral biofilm and influences the electrochemical behavior of titanium: An in situ and in vitro study. Journal of Periodontology, v. 90, n. 2, p. 149-158, FEB 2019. (15/23118-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, João Gabriel Silva. Microorganisms interaction in oral biofilms and effect of surface treatment on titanium to reduce microbial accumulation. 2019. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.