Evaluating the effects of blue and red light on the stress tolerance and the proteome of the entomopathogenic fungus Metarhizium acridum

• Grant number: 15/24305-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2016
• End date: July 01, 2019
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Gilberto Úbida Leite Braga
• Grantee: Guilherme Thomaz Pereira Brancini
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 18/00355-7 - The Neurospora crassa methyltransferase LAE-1: molecular function and interacting partners, BE.EP.DR

Abstract

Light response is a wide studied phenomenon in some fungi, such as Neurospora crassa and Aspergillus nidulans. However, the vast majority of fungal species have not had their light response mechanism elucidated. In the entomopathogenic fungus Metarhizium acridum, light is responsible for inducing an increase in tolerance to UV-B (290-315 nm) radiation. Given the potential use of this fungus for the biological control of insects, this increased tolerance is a high-valued characteristic, as one of the most important limitations of using fungi in biological control is inactivation due to solar radiation, particularly ultraviolet radiation. Even though induction of UV-B tolerance is a known effect of light in M. acridum, the molecular mechanism resulting in this increased tolerance has not been determined. Therefore, a proteomic approach combined to obtaining mutants for the main photoreceptors should provide knowledge for a better understanding of light responses in this fungus. We hope that the results gathered here will provide good information for a mechanistic evaluation of light responses and could also be used for producing strains with increased tolerance to UV-B radiation to be employed in biological control.

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARAUJO, CLAUDINEIA A. S.; DIAS, LUCIANA P.; FERREIRA, PAULO C.; MITTMANN, JOSANE; PUPIN, BRENO; BRANCINI, GUILHERME T. P.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N.. Responses of entomopathogenic fungi to the mutagen 4-nitroquinoline 1-oxide. FUNGAL BIOLOGY, v. 122, n. 6, SI, p. 621-628, JUN 2018. (15/24305-0, 13/25964-2, 16/11386-5, 12/15204-8, 13/50518-6, 10/06374-1)

BRANCINI, GUILHERME T. P.; BACHMANN, LUCIANO; FERREIRA, MARCIA ELIANA DA S.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L.. Exposing Metarhizium acridum mycelium to visible light up-regulates a photolyase gene and increases photoreactivating ability. Journal of Invertebrate Pathology, v. 152, p. 35-37, FEB 2018. (15/24305-0, 14/10628-0, 16/11386-5, 13/50518-6, 10/06374-1)

CARDOSO ULIANA, JOAO VITOR; PEREIRA BRANCINI, GUILHERME THOMAZ; CASTELLI-GAIR HOMBRIA, JAMES; DIGIAMPIETRI, LUCIANO ANTONIO; ANDRIOLI, LUIZ PAULO; MONESI, NADIA. Characterizing the embryonic development of B. hygida (Diptera: Sciaridae) following enzymatic treatment to permeabilize the serosal cuticle. MECHANISMS OF DEVELOPMENT, v. 154, p. 270-276, DEC 2018. (16/25325-8, 16/00412-5, 15/24305-0, 14/14318-5)

ALVARENGA, VERONICA O.; BRANCINI, GUILHERME T. P.; SILVA, ERIC K.; DA PIA, ARTHUR K. R.; CAMPAGNOLLO, FERNANDA B.; BRAGA, GILBERTO U. L.; HUBINGER, MIRIAM D.; SANT'ANA, ANDERSON S.. Survival variability of 12 strains of Bacillus cereus yielded to spray drying of whole milk. International Journal of Food Microbiology, v. 286, p. 80-89, DEC 2 2018. (16/11386-5, 15/24305-0, 14/14891-7)

BRANCINI, GUILHERME T. P.; FERREIRA, MARCIA E. S.; RANGEL, DRAUZIO E. N.; BRAGA, GILBERTO U. L.. Combining Transcriptomics and Proteomics Reveals Potential Post-transcriptional Control of Gene Expression After Light Exposure in Metarhizium acridum. G3-GENES, GENOMES, GENETICS, v. 9, n. 9, p. 2951-2961, SEP 2019. (15/24305-0, 16/11386-5, 13/50518-6, 10/06374-1)

ALDER-RANGEL, ALENE; IDNURM, ALEXANDER; BRAND, ALEXANDRA C.; BROWN, ALISTAIR J. P.; GORBUSHINA, ANNA; KELLIHER, CHRISTINA M.; CAMPOS, CLAUDIA B.; LEVIN, DAVID E.; BELL-PEDERSEN, DEBORAH; DADACHOVA, EKATERINA; et al. The Third International Symposium on Fungal Stress - ISFUS. FUNGAL BIOLOGY, v. 124, n. 5, p. 235-252, MAY 2020. (15/24305-0, 16/11386-5, 18/17172-2, 17/22669-0, 13/50518-6, 18/20571-6, 10/06374-1, 16/14542-8, 14/01229-4)

RODRIGUES, GABRIELA BRAGA; PEREIRA BRANCINI, GUILHERME THOMAZ; UYEMURA, SERGIO AKIRA; BACHMANN, LUCIANO; WAINWRIGHT, MARK; LEITE BRAGA, GILBERTO UBIDA. Chemical features of the photosensitizers new methylene blue N and S137 influence their subcellular localization and photoinactivation efficiency in Candida albicans. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 209, AUG 2020. (12/15204-8, 12/22933-6, 15/24305-0)

ARAUJO, CLAUDINEIA A. S.; DIAS, LUCIANA P.; FERREIRA, PAULO C.; MITTMANN, JOSANE; PUPIN, BRENO; BRANCINI, GUILHERME T. P.; BRAGA, GILBERTO U. L.; RANGEL, DRAUZIO E. N.. Responses of entomopathogenic fungi to the mutagen 4-nitroquinoline 1-oxide. FUNGAL BIOLOGY, v. 122, n. 6, p. 8-pg., 2018-06-01. (10/06374-1, 13/25964-2, 12/15204-8, 13/50518-6, 16/11386-5, 15/24305-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BRANCINI, Guilherme Thomaz Pereira. The photobiology of Metarhizium acridum: light quality, stress tolerance, and gene regulation. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) Ribeirão Preto.