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Structural and functional aspects of two DNA binding proteins encoded by Corynebacterium pseudotuberculosis

Grant number: 16/08104-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2016
Effective date (End): June 30, 2018
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Raghuvir Krishnaswamy Arni
Grantee:Raphael Josef Eberle
Home Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil

Abstract

DNA as the molecule that carries the biological code and the information about all proteins in the cell is highly sensitive against mutations that may result in cell death. The proteins MutY and MuG are part of the base excision repair (BER) pathway which protects the DNA from mutations. Our interest is to understand the specificity of this mechanism, the molecular and structural basics behind and even the inhibition of this function. Furthermore, the design and development of inhibitor lead structures.This pathogen has an importance in the global economy with large losses in the production of meat, milk and wool production. Several countries have had losses of millions of dollars a year, such as the Brazilian Northeast, which has the largest goat herd in Brazil. And Australia, which had in a year, losses of 35 million dollars.Structural research, biophysics and biochemistry can provide important information about the druggability of the targets under investigation.Functional and structural research of proteins is a key feature of Multi-User Centre for Bio molecular Innovation (CMIB) located in the state university of São Paulo-UNESP (São José do Rio Preto) (http://www.cmib-unesp.com/). (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEINADO, RAFAELA DOS S.; OLIVIER, DANILO S.; EBERLE, RAPHAEL J.; DE MORAES, FABIO R.; AMARAL, MARCOS S.; ARNI, RAGHUVIR K.; CORONADO, MONIKA A. Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B-12 Metabolism. SCIENTIFIC REPORTS, v. 9, APR 23 2019. Web of Science Citations: 0.
EBERLE, RAPHAEL J.; CORONADO, MONIKA A.; PEINADO, RAFAELA S.; DE MORAES, FABIO R.; OLIVIER, DANILO; DREYER, THIAGO; LOPES, DEBORA DE OLIVEIRA; ROSA DA LUZ, BRENDA SILVA; AZEVEDO, VASCO; ARNI, RAGHUVIR K. The polyanions heparin and suramin impede binding of free adenine to a DNA glycosylase from C. pseudotuberculosis. International Journal of Biological Macromolecules, v. 125, p. 459-468, MAR 15 2019. Web of Science Citations: 0.
CORONADO, MONIKA APARECIDA; EBERLE, RAPHAEL JOSEF; BLEFFERT, NICOLE; FEUERSTEIN, SOPHIE; OLIVIER, DANILO SILVA; DE MORAES, FABIO ROGERIO; WILLBOLD, DIETER; ARNI, RAGHUVIR KRISHNASWAMY. Zika virus NS2B/NS3 proteinase: A new target for an old drug - Suramin a lead compound for NS2B/NS3 proteinase inhibition. Antiviral Research, v. 160, p. 118-125, DEC 2018. Web of Science Citations: 6.
EBERLE, RAPHAEL J.; KAWAI, LIEGE A.; DE MORAES, FABIO R.; OLIVIER, DANILO; DO AMARAL, MARCOS S.; TASIC, LJUBICA; AMI, RAGHUVIR K.; CORONADO, MONIKA A. Inhibition of thioredoxin A1 from Corynebacterium pseudotuberculosis by polyanions and flavonoids. International Journal of Biological Macromolecules, v. 117, p. 1066-1073, OCT 1 2018. Web of Science Citations: 0.
CORONADO, MONIKA APARECIDA; OLIVIER, DANILO DA SILVA; EBERLE, RAPHAEL JOSEF; DO AMARAL, MARCOS SERROU; ARNI, RAGHUVIR KRISHNASWAMY. Modeling and molecular dynamics indicate that snake venom phospholipase B-like enzymes are Ntn-hydrolases. Toxicon, v. 153, p. 106-113, OCT 2018. Web of Science Citations: 1.
EBERLE, RAPHAEL J.; KAWAI, LIEGE A.; DE MORAES, FABIO R.; TASIC, LJUBICA; ARNI, RAGHUVIR K.; CORONADO, MONIKA A. Biochemical and biophysical characterization of a mycoredoxin protein glutaredoxin A1 from Corynebacterium pseudotuberculosis. International Journal of Biological Macromolecules, v. 107, n. B, p. 1999-2007, FEB 2018. Web of Science Citations: 1.
CORONADO, MONIKA A.; CARUSO, ICARO P.; DE OLIVEIRA, VINICIUS M.; CONTESSOTO, VINICIUS G.; LEITE, VITOR B. P.; KAWAI, LIEGE A.; ARNI, RAGHUVIR K.; EBERLE, RAPHAEL J. Cold Shock Protein A from Corynebacterium pseudotuberculosis: Role of Electrostatic Forces in the Stability of the Secondary Structure. PROTEIN AND PEPTIDE LETTERS, v. 24, n. 4, p. 358-367, 2017. Web of Science Citations: 6.

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