Interação da toxina Cry1Ac de Bacillus thurigiensis às microvilosidades apicais das células colunares (BBMVs) do intestino médio de diferentes ínstares larvais de Helicoverpa armigera (Lepidoptera: Noctuidae)

• Grant number: 15/24330-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: July 01, 2016
• End date: July 31, 2017
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Ricardo Antonio Polanczyk
• Grantee: Igor Henrique Sena da Silva
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated scholarship(s): 16/21464-3 - Bacillus thuringiensis Cry1Ac interactions to brush border emebrane vesicles (BBMV) in the midgut of different larval instars of Helicoverpa armigera (Lepidoptera: Noctuidae), BE.EP.MS

Abstract

Helicoverpa armigera is one of the most important pest species in Brazil because attack many valuable crops causing losses until U$ 2 billion in one season. Duo of the intensive use of pesticides to control this pest, populations has developed resistance to various pesticides, and this species is the one with the higher number of reports of pest resistance to pesticides. Thus, it is necessary to use other forms to control this pest, for example, microbial control with the use of the Bacillus thuringiensis bacterium (Bt), that besides its efficacy its innocuous to the environment and natural enemies. This bacteria has been used in several countries to control this pest, as biological insecticides or transgenic plants. Among the assayed toxins against H. armigera, Cry1Ac toxin stands out with the virulent to this insect. However, there are several reports of differences in H. armigera Cry1Ac toxin susceptibility to different larval instars. This susceptibility variation to Cry1Ac toxin can be associated to changes in the toxins interaction to membrane receptors in the brush border membrane vesicles (BBMVs) of the midgut to, such as the Cry toxin binding capacity to receptor (reversible binding), or the expression of these receptors in the membrane. Thus, the aims of this project is to evaluate the toxicity to Cry1Ac toxin from Bt to H. armigera in different instars, to correlate the insect susceptibility to toxin binding capacity and to identify and quantify the receptors to Cry1Ac in different instars of this pest as well. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SENA DA SILVA, IGOR HENRIQUE; GOMEZ, ISABEL; SANCHEZ, JORGE; MARTINEZ DE CASTRO, DIANA L.; VALICENTE, FERNANDO HERCOS; SOBERON, MARIO; POLANCZYK, RICARDO ANTONIO; BRAVO, ALEJANDRA. Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin. PLoS One, v. 13, n. 12, DEC 6 2018. (15/24330-5, 16/21464-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Igor Henrique Sena da. Interaction of Cry1ac toxin from Bacillus thuringiensis to brush border membrane of Helicoverpa armigera Hübner, 1805 (Lepidoptera: Noctuidae) midgut in different larval instars. 2017. Master's Dissertation - Universidade Estadual Paulista (Unesp).