Analysis of autophagy and the role of co-chaperone bag-2 upon protein aggregates in neurodegenerative diseases

• Grant number: 16/04409-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2016
• End date: January 31, 2019
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Merari de Fátima Ramires Ferrari
• Grantee: Raquel de Souza Lima
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated scholarship(s): 17/24722-6 - Chaperones machines to combat protein aggregation in Parkinson's Disease, BE.EP.MS

Abstract

Aging is a worldwide issue and the understanding of the cellular events associated to this process are important for the study of age-related diseases, like in neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. The presence of misfolded proteins are common during the neurodegenerative process, and can contribute to organelle dysfunction culminating in cell death. In view of this, the purpose of the present study is to evaluate autophagy process during the course of sporadic neurodegenerative diseases. In view of this, it is proposed to evaluate the protein levels of Beclin-1, LC3I and LC3II, associated with the recruitment and maintenance of the autophagosomes, respectively, and autophagy flux, in culture cells of hippocampus, substantia nigra and locus coeruleus exposed to rotenone as a model of protein aggregation. Also it is intended to analyze the role of the co-chaperon BAG-2, that transits between the lysosomal and proteasome systems, to modulate the autophagic activity and protein aggregation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, RAQUEL S.; CARRETTIERO, DANIEL C.; FERRARI, MERARI F. R.. BAG2 prevents Tau hyperphosphorylation and increases p62/SQSTM1 in cell models of neurodegeneration. MOLECULAR BIOLOGY REPORTS, v. N/A, p. 13-pg., 2022-05-25. (11/06434-7, 13/08028-1, 18/07592-4, 17/24722-6, 16/04409-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LIMA, Raquel de Souza. Effects of BAG2 and DNAJB6 chaperones on protein degradation in cell models of neurodegenerative diseases. 2019. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.