Study of Detoxification Enzymes Interaction with chemotherapeutic drugs and its relationship with chemoresistance

• Grant number: 16/00991-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2016
• End date: July 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Ronaldo Censi Faria
• Grantee: Elsa María Materón Vásques
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated scholarship(s): 18/00749-5 - Biosensor to detect chemotherapeutic drugs related to chemoresistance, BE.EP.PD

Abstract

The therapeutic efficacy of drugs widely used in cancer treatments for a range of different types of cancerous tumor and other pathologies are found to be affected by intrinsic or acquired resistance that may develop during the treatment, and this has in essence become one of the most crucial problems of medicine, more specifically in therapy against cancer. It is in this light that the development of analytical methods aiming at monitoring the chemotherapy resistance and consequently the monitoring of the effectiveness of anticancer drugs stand to be of imperative relevance and of great interest once they play a major role in improving clinical treatments. This can be attained through the study of the action mechanism and the properties of detoxification enzymes that can be used in devices for the detection of problems related to drug metabolism. Therefore, the overall objective of the project lies in studying the action mechanism of detoxification enzymes such as metalotionenina, cytochrome P450, and glutathione-S-transferase present in the metabolism of neoplastic drugs, and this knowledge once acquired will be implemented in the simultaneous detection of platinum compounds (cisplatin), methotrexate, tamoxifen, doxorubicin and gemcitabine in modified screen-printed multichannel electrodes and field-effect transistors and thus contributing towards the improvement of chemotherapy treatments.

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATERON, ELSA M.; MIYAZAKI, CELINA M.; CARR, OLIVIA; JOSHI, NIRAV; PICCIANI, PAULO H. S.; DALMASCHIO, CLEOCIR J.; DAVIS, FRANK; SHIMIZU, FLAVIO M.. Magnetic nanoparticles in biomedical applications: A review. APPLIED SURFACE SCIENCE ADVANCES, v. 6, DEC 2021. (18/22214-6, 14/23546-1, 16/00991-5)

PROENCA, CAMILA A.; BALDO, THAISA A.; FREITAS, TAYANE A.; MATERON, ELSA M.; WONG, ADEMAR; DURAN, ANDRES A.; MELENDEZ, MATIAS E.; ZAMBRANO, GUSTAVO; FARIA, RONALDO C.. Novel enzyme-free immunomagnetic microfluidic device based on Co0.25Zn0.75Fe2O4 for cancer biomarker detection. Analytica Chimica Acta, v. 1071, p. 59-69, SEP 13 2019. (17/24053-7, 16/00991-5, 15/19890-1)

MATERON, ELSA MARIA; MARCHETTO, REINALDO; ARAUJO, ANGELA REGINA; VEGA-CHACON, JAIME; PIVIDORI, I, MARIA; JAFELICCI JR, MIGUEL; SHIMIZU, FLAVIO M.; OLIVEIRA JR, OSVALDO N.; BOLDRIN ZANONI, MARIA VALNICE. A simple electrochemical method to monitor an azo dye reaction with a liver protein. Analytical Biochemistry, v. 553, p. 46-53, JUL 15 2018. (13/14262-7, 12/15543-7, 16/00991-5)

PROENCA, CAMILA A.; FREITAS, TAYANE A.; BALDO, THAISA A.; MATERON, ELSA M.; SHIMIZU, FLAVIO M.; FERREIRA, GABRIELLA R.; SOARES, FREDERICO L. F.; FARIA, RONALDO C.; OLIVEIRA JR, OSVALDO N.. Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors. BEILSTEIN JOURNAL OF NANOTECHNOLOGY, v. 10, p. 2171-2181, NOV 6 2019. (13/14262-7, 16/00991-5)

DOS SANTOS, KEVIN F.; MATERON, ELSA M.; OLIVEIRA JR, OSVALDO N.. Influence of cytochrome P450 3A4 and membrane lipid composition on doxorubicin activity. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 220, p. 9-pg., 2022-12-01. (16/00991-5)

FREITAS, TAYANE A.; PROENCA, CAMILA A.; BALDO, THAISA A.; MATERON, ELSA M.; WONG, ADEMAR; MAGNANI, RODRIGO F.; FARIA, RONALDO C.. Ultrasensitive immunoassay for detection of Citrus tristeza virus in citrus sample using disposable microfluidic electrochemical device. Talanta, v. 205, DEC 1 2019. (17/24053-7, 16/00991-5, 15/19890-1)

MATERON, ELSA M.; SHIMIZU, FLAVIO M.; DOS SANTOS, KEVIN FIGUEIREDO; NASCIMENTO, GUSTAVO F.; GERALDO, VANANELIA P. N.; OLIVEIRA JR, OSVALDO N.; FARIA, RONALDO C.. Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance. BIOMEDICINE & PHARMACOTHERAPY, v. 145, JAN 2022. (12/15543-7, 17/24053-7, 16/00991-5, 18/22214-6)

IBANEZ-REDIN, GISELA; FURUTA, ROBERTO H. M.; WILSON, DEIVY; SHIMIZU, FLAVIO M.; MATERON, ELSA M.; REBOLHO BATISTA ARANTES, LIDIA MARIA; MELENDEZ, MATIAS E.; CARVALHO, ANDRE L.; REIS, RUI MANUEL; CHAUR, MANUEL N.; et al. Screen-printed interdigitated electrodes modified with nanostructured carbon nano-onion films for detecting the cancer biomarker CA19-9. Materials Science & Engineering C-Materials for Biological Applications, v. 99, p. 1502-1508, JUN 2019. (17/12096-3, 13/14262-7, 15/01770-0, 16/00991-5, 12/15543-7)

BALDO, THAISA APARECIDA; PROENCA, CAMILA DOS ANJOS; FELIX, FABIANA DA SILVA; FREITAS, TAYANE AGUIAR; SAKATA, SOLANGE KAZUMI; ANGNES, LUCIO; FARIA, RONALDO CENSI. Disposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples. Talanta, v. 232, SEP 1 2021. (13/14262-7, 17/24053-7, 16/00991-5, 14/50867-3, 17/13137-5)

MEJIA-SALAZAR, JORGE RICARDO; CRUZ, KAMILLA RODRIGUES; MATERON VASQUES, ELSA MARIA; DE OLIVEIRA, JR., OSVALDO NOVAIS. Microfluidic Point-of-Care Devices: New Trends and Future Prospects for eHealth Diagnostics. SENSORS, v. 20, n. 7, APR 2020. (13/14262-7, 16/00991-5)