Evaluation of the role of estrogen in the growth and development of the maxilla and mandible

• Grant number: 16/13982-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2016
• End date: July 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Erika Calvano Kuchler
• Grantee: Marjorie Ayumi Omori
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 15/06866-5 - Evaluation of the role of estrogen in dentofacial development, AP.JP

Abstract

Genetic polymorphisms in genes expressed during craniofacial development are associated with dimensional changes in the maxilla and mandible. In this way, the objective will be to evaluate, using animal models and human genetics, the role of estrogen and genetic polymorphisms in its receptors (ER± and ER²), in the dimensional alterations of the maxilla and the mandible. Ovarectomized Wistar rats will be used as animal model for estrogen deficiency, divided into 2 groups (I-Ovarectomized; II-Sham. Radiographic shots of the craniofacial region of the rats will be performed, the first radiographic will be performed at 20 days, the second at 45 days and the last at 90 days. Cephalometric measurements of the maxilla and mandible will be performed and the cephalometric points and planes will be obtained for the evaluation of vertical and sagittal skeletal alterations. After euthanasia, the hemicranium will be used to perform immunohistochemistry and real-time PCR for ER± and ER². A total of 300 genomic DNA samples and cephalometric analysis data will be evaluated. Genomic DNA samples will be evaluated for genetic polymorphisms by the real-time PCR allele discrimination method. Continuous variables will be evaluated with parametric and / or non-parametric tests to compare means between groups. Fisher's exact chi-square tests will be used to evaluate the association of the facial skeletal pattern with the genotypic distribution and allelic distribution in the human population (5% alpha). (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CALIXTO, ROBSON DIEGO; OMORY, MARJORIE AYUMI; RAMAZZOTTO, LUCAS ALEXANDRE; MADALENA, ISABELA RIBEIRO; HUEB DE MENEZES OLIVEIRA, MARIA ANGELICA; DONADELLI NABARRO, HUMBERTO MADSON; MORO, ALEXANDRE; NELSON-FILHO, PAULO; SCARIOT, RAFAELA; KUCHLER, ERIKA CALVANO. The role of hypoestrogenism on Mmp-8 and Mmp-13 gene expression in maxilla and mandible's growth sites. CLINICAL AND INVESTIGATIVE ORTHODONTICS, v. 81, n. 1, p. 8-pg., 2022-01-02. (16/13982-4)

OMORI, MARJORIE AYUMI; MARANON-VASQUEZ, GUIDO ARTEMIO; ROMUALDO, PRISCILLA COUTINHO; NETO, EVANDRO CARNEIRO MARTINS; STUANI, MARIA BERNADETE SASSO; MATSUMOTO, MIRIAN AIKO NAKANE; NELSON-FILHO, PAULO; PROFF, PETER; LEON, JORGE ESQUICHE; KIRSCHNECK, CHRISTIAN; et al. Effect of ovariectomy on maxilla and mandible dimensions of female rats. ORTHODONTICS & CRANIOFACIAL RESEARCH, v. 23, n. 3, APR 2020. (16/08149-1, 16/13982-4, 15/06866-5)

KUCHLER, ERIKA CALVANO; DO NASCIMENTO, MARIELE ANDRADE; NAKANE MATSUMOTO, MIRIAN AIKO; ROMANO, FABIO LOURENCO; BEZERRA DA SILVA, RAQUEL ASSED; OMORI, MARJORIE AYUMI; ANTUNES, LIVIA AZEREDO; ANTUNES, LEONARDO SANTOS; BEZERRA DA SILVA, LEA ASSED; NELSON-FILHO, PAULO. Genetic polymorphism in RANK is associated with mandibular size. JOURNAL OF ORTHODONTICS, v. 45, n. 3, p. 157-162, 2018. (16/08149-1, 16/13982-4, 15/06866-5)

OMORI, MARJORIE AYUMI; GERBER, JENNIFER TSI; MARANON-VASQUEZ, GUIDO ARTEMIO; NAKANE MATSUMOTO, MIRIAN AIKO; WEISS, SUYANY GABRIELY; NASCIMENTO, MARIELE ANDRADE; DE SOUZA ARAUJO, MONICA TIRRE; SASSO STUANI, MARIA BERNADETE; NELSON-FILHO, PAULO; SCARIOT, RAFAELA; et al. Possible association between craniofacial dimensions and genetic markers in ESR1 and ESR2. JOURNAL OF ORTHODONTICS, v. 47, n. 1, p. 65-71, MAR 2020. (16/08149-1, 16/13982-4, 15/06866-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OMORI, Marjorie Ayumi. Evaluation of the hole of estrogen in the maxilla and mandible growth and development. 2018. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.