Gellan/retrograded starch mucoadhesives systems intended for the colonic release of bevacizumab to cancer treatment.

• Grant number: 15/21412-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2016
• End date: December 31, 2018
• Field of knowledge: Health Sciences - Pharmacy - Pharmaceutical Technology
• Principal Investigator: Maria Palmira Daflon Gremião
• Grantee: Valéria Maria de Oliveira Cardoso
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Colorectal cancer is the third highest incidence among men and women worldwide. Intravenous chemotherapy is the therapeutic alternative most commonly used in the treatment against of the most types of cancers. However, their effectiveness is limited due to lack of specificity/selectivity of chemotherapeutic agents necessitates that makes necessary the high doses administration, leading severe systemic side effects. Bevacizumab was the first monoclonal antibody approved by the FDA for anticancer therapy, and their effectiveness has been demonstrated in the treatment of several solid tumors types. The colon specific release of bevacizumab, by the oral administration of nanostructured systems, represents a promising strategy to increase its specificity action and, subsequent antitumor activity in colon cancer. The bio/mucoadhesive propertie and specific enzymatic degradability of resistant starch and gellan makes them promising materials for the development of colon specific drug release systems. The bevavizumabe delivery for colon in polymeric nanocarriers systems is a rational strategy to increase the biological interaction with the tumoral tissue. Resistant starch and gellan nanoparticles containing bevacizumab will be prepared by polyelectrolytic complexation and characterized by the association efficiency analysis, size, shape, zeta potential, circular dichroism, rheological and thermal properties (DSC and TG/DTG). Cell permeability will be evaluate in Caco-2 cell monolayers. The performance of the nanoparticles as bevacizumab controlled release systems will be evaluated by the vitro release study, in media with pH values that resembles the GIT different portions, and followed by determination of the drug release mechanisms.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VICTORELLI, FRANCESCA DAMIANI; DE OLIVEIRA CARDOSO, VALERIA MARIA; FERREIRA, NATALIA NORONHA; FIORAMONTI CALIXTO, GIOVANA MARIA; FONTANA, CARLA RAQUEL; BALTAZAR, FATIMA; DAFLON GREMIAO, MARIA PALMIRA; CHORILLI, MARLUS. Chick embryo chorioallantoic membrane as a suitable in vivo model to evaluate drug delivery systems for cancer treatment: A review. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 153, p. 273-284, AUG 2020. (14/50928-2, 19/07245-5, 18/04546-1, 13/01565-1, 16/09671-3, 15/21412-0, 16/06337-5, 17/10016-2, 17/23357-2)

VICTORELLI, FRANCESCA DAMIANI; DE OLIVEIRA CARDOSO, VALERIA MARIA; FERREIRA, NATALIA NORONHA; FIORAMONTI CALIXTO, GIOVANA MARIA; FONTANA, CARLA RAQUEL; BALTAZAR, FATIMA; DAFLON GREMIAO, MARIA PALMIRA; CHORILLI, MARLUS. Chick embryo chorioallantoic membrane as a suitable in vivo model to evaluate drug delivery systems for cancer treatment: A review. EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v. 153, p. 12-pg., 2020-08-01. (17/10016-2, 17/23357-2, 19/07245-5, 13/01565-1, 18/04546-1, 16/06337-5, 15/21412-0, 14/50928-2, 16/09671-3)

DE OLIVEIRA CARDOSO, VALERIA MARIA; DAFLON GREMIAO, MARIA PALMIRA; FERREIRA CURY, BEATRIZ STRINGHETTI. Mucin-polysaccharide interactions: A rheological approach to evaluate the effect of pH on the mucoadhesive properties. International Journal of Biological Macromolecules, v. 149, p. 234-245, APR 15 2020. (17/16324-0, 14/50928-2, 15/21412-0)

DE OLIVEIRA CARDOSO, VALERIA MARIA; PEREIRA DE BRITO, NATALIA ARAUJO; FERREIRA, NATALIA NORONHA; BONI, FERNANDA ISADORA; BARBOZA FERREIRA, LEONARDO MIZIARA; CARVALHO, SUZANA GONCALVES; DAFLON GREMIAO, MARIA PALMIRA. Design of mucoadhesive gellan gum and chitosan nanoparticles intended for colon-specific delivery of peptide drugs. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 628, NOV 5 2021. (14/50928-2, 16/09671-3, 15/21412-0, 18/04546-1)

CARDOSO, VALERIA MARIA DE OLIVEIRA; KIRALY, VANESSA THOMAZ RODRIGUES; BONI, FERNANDA ISADORA; FERREIRA, NATALIA NORONHA; FERREIRA, LEONARDO M. B.; PEREIRA, FABIOLA MANHAS VERBI; BORGES, JULIO CESAR; CURY, BEATRIZ STRINGHETTI FERREIRA; GREMIAO, MARIA PALMIRA DAFLON. Rational design of nanocarriers based on gellan gum/retrograded starch exploiting polyelectrolyte complexation and ionic cross-linking processes: A potential technological platform for oral delivery of bevacizumab. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY, v. 66, DEC 2021. (17/16324-0, 14/50928-2, 15/21412-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CARDOSO, Valéria Maria de Oliveira. Sistemas mucoadesivos de gelana/amido retrogradado destinados à liberação colônica do bevacizumabe para o tratamento do câncer colorretal. 2019. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.