Proteomic analysis of the jejunum and ileum in rats exposed to chronic or acute doses of fluoride.

• Grant number: 16/09100-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2016
• End date: June 30, 2020
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: Aline Dionizio Valle
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil

Abstract

Because the gastrointestinal tract is regarded as the primary route of exposure to fluoride (F), which is rapidly absorbed from it, it is believed that exposure to this element can lead to significant changes in the intestinal morphology, which can affect its function. In a previous work by our research group it was observed that exposure to F significantly interferes with the expression of several proteins of the duodenum. In addition, exposure to 50 mg/L F caused a significant decrease in the density of the overall population of neurons of the jejunum and ileum and density of immunoreactive neurons nNOS in jejunum. Thus, this study aims to evaluate the effect of exposure to acute or chronic doses of F in the proteomic profile of the ileum and jejunum. Male Wistar rats (60 days old) will be treated for 30 days with chronic doses of 0 mg/L F, 10 mg/L F or 50 mg/L F, while the acute dose will be 0 or 25mgF/kg (gastric gavage). After the experimental period, the animals will be anesthetized with 10 mg/kg body weight Anasedan + 100mg/kg of ketamine hydrochloride (Vetaset) for the collection of the ileum and jejunum. Proteomic analysis of the jejunum and ileum will be conducted in order that we can better understand the mechanisms involved in acute and chronic intoxication F by, which lead to the morphological changes observed in the previous study of our research group.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DIONIZIO, ALINE; MELO, CARINA GUIMARAES SOUZA; SABINO-ARIAS, ISABELA TOMAZINI; ARAUJO, TAMARA TEODORO; VENTURA, TALITA MENDES OLIVEIRA; LEITE, ALINE LIMA; SOUZA, SARA RAQUEL GARCIA; SANTOS, ERIKA XAVIER; HEUBEL, ALESSANDRO DOMINGUES; SOUZA, JULIANA GADELHA; et al. Effects of acute fluoride exposure on the jejunum and ileum of rats: Insights from proteomic and enteric innervation analysis. Science of The Total Environment, v. 741, NOV 1 2020. (11/10233-7, 16/09100-6, 12/16840-5)

DIONIZIO, ALINE; UYGHURTURK, DAWUD ABDUWELI; SOUZA MELO, CARINA GUIMARAES; SABINO-ARIAS, ISABELA TOMAZINI; ARAUJO, TAMARA TEODORO; SILVA VENTURA, TALITA MENDES; COLOMBO MARTINS PERLES, JULIANA VANESSA; ZANONI, JACQUELINE NELISIS; DEN BESTEN, PAMELA; RABELO BUZALAF, MARILIA AFONSO. Intestinal changes associated with fluoride exposure in rats: Integrative morphological, proteomic and microbiome analyses. Chemosphere, v. 273, JUN 2021. (11/10233-7, 16/09100-6, 12/16840-5)

DIONIZIO, ALINE SALGADO; SOUZA MELO, CARINA GUIMARAES; SABINO-ARIAS, ISABELA TOMAZINI; SILVA VENTURA, TALITA MENDES; LEITE, ALINE LIMA; GARCIA SOUZA, SARA RAQUEL; SANTOS, ERIKA XAVIER; HEUBEL, ALESSANDRO DOMINGUES; SOUZA, JULIANA GADELHA; COLOMBO MARTINS PERLES, JULIANA VANESSA; et al. Chronic treatment with fluoride affects the jejunum: insights from proteomics and enteric innervation analysis. SCIENTIFIC REPORTS, v. 8, FEB 16 2018. (16/09100-6, 11/10233-7, 12/16840-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VALLE, Aline Dionizio. Proteomic analysis of jejunum and ileum in rats exposed to acute or chronic fluoride dose. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.