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The use of low-intensity ultrasound, parathyroid hormone or sodium alendronate on the bone healing of femurs in rats with spinal cord injury

Grant number: 16/13026-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2016
Effective date (End): November 30, 2018
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:José Batista Volpon
Grantee:Mariana Maloste Butezloff
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Background: Fracture is a frequent event in the spinal cord injured patient and it occurs because of the marked reduction in mass and bone quality as experienced by those individuals, also compromising the reparative process which ensues a poor quality bone callus or even union anomalies. Therefore, it is interesting to search for therapeutic processes that can improve bone healing in this population. Purpose: To evaluate the therapeutic results with sodium alendronate, parathyroid hormone or low intensity ultrasound on the quality of bone and fracture callus. Material and Methods: (n=15/group) (1) normal rats subjected to fracture; (2) rats with spinal cord injury and fracture; (3) rats with spinal cord injury, fracture and treatment with ALE; (4) rats with spinal cord injury, fracture and treatment with PTH, and (5) rats with spinal cord injury, fracture and treatment with LIPUS. The spinal cord will be completely transected under direct vision after surgical exposition and at the T10 level. In the control group the spinal cord will be exposed, but not injured. The fracture will be created at de middle of the femur (diaphysis) 10 days after the spinal cord injury and fixed with a steel wire placed in the medullary canal. The specific therapy will be applied during the next two weeks of fracture production. The bone quality and callus assessment will be made by µCT, mechanical strength and optic microscopy (immunohistochemistry with markers for bone formation and resorption), biochemical analysis of bone formation, resorption (anti-RANK and anti-RANKL, OPG anti-sclerostin and anti-OC) and adiponectin levels. Gene expression will also be analyzed by real-time PCR.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BUTEZLOFF, Mariana Maloste. The use of ultrasound of low intensity, parathyroid hormone or alendronate sodium in bone healing of femoral fractures in spinal cord injured rats. 2018. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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